Anti-TMS1/ASC 抗体 (ab180799)
Key features and details
- Rabbit polyclonal to TMS1/ASC
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMS1/ASC antibody
TMS1/ASC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TMS1/ASC -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein corresponding to Human TMS1/ASC aa 1-195.
Sequence:MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDAL DLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGI QAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVR AEPTNPSKMRKLFSFTPAWNWTCKDLLLQA LRESQSYLVEDLERS
Database link: Q9ULZ3 -
ポジティブ･コントロール
- A549, THP1, HT29 and BT474 cell line extracts.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180799の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/500 - 1/2000. Predicted molecular weight: 22 kDa.
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| 特記事項 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/500 - 1/2000. Predicted molecular weight: 22 kDa. |
ターゲット情報
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機能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
組織特異性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
配列類似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
ドメイン
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻訳後修飾
Phosphorylated. -
細胞内局在
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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参照データベース
- Entrez Gene: 29108 Human
- Entrez Gene: 282817 Rat
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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別名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
画像
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Western blot - Anti-TMS1/ASC antibody (ab180799)All lanes : Anti-TMS1/ASC antibody (ab180799) at 1/500 dilution
Lane 1 : A549 cell line extract
Lane 2 : THP1 cell line extract
Lane 3 : HT29 cell line extract
Lane 4 : BT474 cell line extract
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab180799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling TMS1/ASC with ab180799 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab180799)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling TMS1/ASC with ab180799 at 1/200. Magnification: 200x.
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Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody (ab180799)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab180799. Blue DAPI for nuclear staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab180799 は 23 報の論文で使用されています。
- Shen J et al. TLR9 regulates NLRP3 inflammasome activation via the NF-kB signaling pathway in diabetic nephropathy. Diabetol Metab Syndr 14:26 (2022). PubMed: 35120573
- Zhang T et al. CircNEIL3 mediates pyroptosis to influence lung adenocarcinoma radiotherapy by upregulating PIF1 through miR-1184 inhibition. Cell Death Dis 13:167 (2022). PubMed: 35190532
- Cai J et al. SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β‑catenin/TCF/LEF signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 35419615
- Gao J et al. Oxymatrine inhibits the pyroptosis in rat insulinoma cells by affecting nuclear factor kappa B and nuclear factor (erythroid-derived 2)-like 2 protein/heme oxygenase-1 pathways. Korean J Physiol Pharmacol 26:165-174 (2022). PubMed: 35477544
- Wu DM et al. Increased susceptibility of irradiated mice to Aspergillus fumigatus infection via NLRP3/GSDMD pathway in pulmonary bronchial epithelia. Cell Commun Signal 20:98 (2022). PubMed: 35761358