Anti-TMS1/ASC 抗体 [EPR28201-61] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat spleen.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Low expression tissue : no staining on rat skeletal muscle.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat colon.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Low expression tissue : no staining on mouse skeletal muscle.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse head and neck tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse head and neck squamous cell carcinoma.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse colon.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) J774A.1 (mouse r tissue labeling TMS1/ASC with ab307560 at 1/1000 (0.484 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cell pellet, no staining on (B) RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellet.The section was incubated with ab307560 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling TMS1/ASC with ab307560 at 1/500 (0.968 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing nuclear and cytoplasmic staining in J774A.1 cell line. Negative control : RAW 264.7 (PMID : 28766178Ј©
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW264.7 (mouse abelson murine leukemia virus-induced tumor macrophage, Left) / J774A.1 (mouse reticulum cell sarcoma monocyte macrophage, Right) cells labelling TMS1/ASC with ab307560 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control : RAW 264.7 (PMID : 28766178).

• IP

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Immunoprecipitation - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using ab307560, the same antibody clone in a different buffer formulation.

TMS1/ASC was immunoprecipitated from 0.35 mg J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 ug with ab307560 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307560 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 ug
Lane 2 : ab307560 IP in J774A.1 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307560 in J774A.1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds

All lanes:

Immunoprecipitation - Anti-TMS1/ASC antibody [EPR28201-61] (<a href='/products/primary-antibodies/tms1-asc-antibody-epr28201-61-ab307560'>ab307560</a>) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage ) whole cell lysate 10 μg

Lane 2:

J774A.1 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 21 kDa

false

Exposure time: 50s

• WB

Supplier Data

Western blot - Anti-TMS1/ASC antibody [EPR28201-61] - BSA and Azide free (AB307561)

This data was developed using 307560, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : RAW 264.7 (PMID : 28766178); Low expression tissue : skeletal muscle (PMID : 32659370). Lanes 1-6 : 15 seconds; Lane 7 : 125 seconds. Exposure time :

All lanes:

Western blot - Anti-TMS1/ASC antibody [EPR28201-61] (<a href='/products/primary-antibodies/tms1-asc-antibody-epr28201-61-ab307560'>ab307560</a>) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticum cell sarcoma monocyte/macrophage ) whole cell lysate 20 μg

Lane 2:

RAW 264.7 (mouse elson murine leukemia virus-induced tumor macrophage) whole cell lysate 20 μg

Lane 3:

Mouse spleen tissue lysate 20 μg

Lane 4:

Mouse colon tissue lysate 20 μg

Lane 5:

Mouse skeletal muscle tissue lysate 20 μg

Lane 6:

Rat spleen tissue lysate 20 μg

Lane 7:

Rat colon tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 21 kDa

false

Exposure time: 15s