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リコンビナントRabMAb

Anti-TMF 抗体 [EPR10671(B)] (ab151702)

Reviews (3) Submit a question References (1)
Western blot - Anti-TMF antibody [EPR10671(B)] (ab151702)
  • Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] (ab151702)
  • Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] (ab151702)
  • Anti-TMF antibody [EPR10671(B)] (ab151702)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10671(B)] to TMF
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TMF antibody [EPR10671(B)]
    TMF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10671(B)] to TMF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human TMF (C terminal).
    Database link: P82094

  • ポジティブ･コントロール

    • HepG2, HeLa, HT1376 cell lysate; MCF7 cells; HT1376 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151702の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF (2)
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Potential coactivator of the androgen receptor. Mediates STAT3 degradation. May play critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. This protein binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP).

  • ドメイン

    The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].

  • 細胞内局在

    Cytoplasm. Nucleus. Golgi apparatus membrane. Concentrated at the budding structures localized at the tips of cisternae.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Androgen receptor coactivator 160 kDa protein antibody
      • Androgen receptor-associated protein of 160 kDa antibody
      • ARA160 antibody
      • TATA element modulatory factor 1 antibody
      • TATA element modulatory factor antibody
      • TMF antibody
      • Tmf1 antibody
      • TMF1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-TMF antibody [EPR10671(B)] (ab151702)
      Western blot - Anti-TMF antibody [EPR10671(B)] (ab151702)
      All lanes : Anti-TMF antibody [EPR10671(B)] (ab151702) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HT1376 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 123 kDa

    • Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] (ab151702)
      Flow Cytometry (Intracellular) - Anti-TMF antibody [EPR10671(B)] (ab151702)

      Intracellular flow cytometric analysis of permeabilized HT1376 cells labeling TMF with ab151702 at 1/10 dilution (red). Rabbit IgG negative control (green). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] (ab151702)
      Immunocytochemistry/ Immunofluorescence - Anti-TMF antibody [EPR10671(B)] (ab151702)

      Immunofluorescent analysis of MCF7 cells labeling TMF with ab151702 at 1/100 dilution.

    • Anti-TMF antibody [EPR10671(B)] (ab151702)
      Anti-TMF antibody [EPR10671(B)] (ab151702)

    参考文献 (1)

    ab151702 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151702 は 1 報の論文で使用されています。

    • Yue X  et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TMF antibody [EPR10671(B)]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mice hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Jul 24 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TMF antibody [EPR10671(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RWPE-1 - prosate epithelial cells)
    Permeabilization
    Yes - 0.2% Triton X-100
    Specification
    RWPE-1 - prosate epithelial cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Jul 19 2019

    Western blot abreview for Anti-TMF antibody [EPR10671(B)]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    20 µg
    Specification
    22Rv1 - prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    投稿 Jul 18 2019

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