Anti-TMEM79 抗体 - N-terminal (ab185453)
Key features and details
- Rabbit polyclonal to TMEM79 - N-terminal
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TMEM79 antibody - N-terminal -
製品の詳細
Rabbit polyclonal to TMEM79 - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human TMEM79 aa 1-100 (N terminal).
Database link: Q9BSE2 -
ポジティブ･コントロール
- IHC-P: Human prostate, skin, liver, testis tissue. WB: RT-4 cell line lysate. ICC/IF: MCF7 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185453の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa.
|
|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 44 kDa. |
|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
-
関連性
TMEM79 is a multi-pass membrane protein. The exact function of TMEM79 remains unknown. -
細胞内局在
Lysosome. Golgi apparatus; trans-Golgi network. Membrane; Multi-pass membrane protein. Note: Colocalized with TGOLN2 in the trans-Golgi network. Colocalized with LAMP1 in the lysosome -
参照データベース
- Entrez Gene: 84283 Human
- Omim: 615531 Human
- SwissProt: Q9BSE2 Human
-
別名
- FLJ16057 antibody
- FLJ32254 antibody
- MGC13102 antibody
see all
画像
-
Western blot - Anti-TMEM79 antibody - N-terminal (ab185453)Anti-TMEM79 antibody - N-terminal (ab185453) at 1/100 dilution + RT-4 cell line lysate
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TMEM79 antibody - N-terminal (ab185453)MCF7 cells stained for TMEM79 (green) using ab185453 at 2 µg/ml dilution in ICC/IF.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM79 antibody - N-terminal (ab185453)Paraffin embedded human skin prostate stained for TMEM79 using ab185453 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM79 antibody - N-terminal (ab185453)Paraffin embedded human testis prostate stained for TMEM79 using ab185453 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM79 antibody - N-terminal (ab185453)Paraffin embedded human skin tissue stained for TMEM79 using ab185453 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM79 antibody - N-terminal (ab185453)Paraffin embedded human liver tissue stained for TMEM79 using ab185453 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab185453 は論文での使用が確認できていません。