Anti-TMEM66/SARAF 抗体 (ab154538)
Key features and details
- Rabbit polyclonal to TMEM66/SARAF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TMEM66/SARAF antibody -
製品の詳細
Rabbit polyclonal to TMEM66/SARAF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Recombinant fragment corresponding to Human TMEM66/SARAF aa 1-211.
Database link: Q96BY9 -
ポジティブ･コントロール
- 293T, Jurkat, Raji whole cell lysate; Human hepatoma tissue; HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154538の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 37 kDa.
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| ICC/IF |
1/100 - 1/1000.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 37 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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組織特異性
Highly expressed in macrophages. -
配列類似性
Belongs to the TMEM66 family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 51669 Human
- Entrez Gene: 67887 Mouse
- Omim: 6147768 Human
- SwissProt: Q96BY9 Human
- SwissProt: Q8R3Q0 Mouse
- Unigene: 521487 Human
- Unigene: 290353 Mouse
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別名
- FLJ22274 antibody
- FOAP 7 antibody
- HBV X transactivated gene 3 protein antibody
see all
画像
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Western blot - Anti-TMEM66/SARAF antibody (ab154538)Anti-TMEM66/SARAF antibody (ab154538) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 37 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM66/SARAF antibody (ab154538)Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling TMEM66/SARAF with ab154538 at 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-TMEM66/SARAF antibody (ab154538)Immunofluorescent analysis of methanol-fixed HeLa cells labeling TMEM66/SARAF with ab154538 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154538 は 1 報の論文で使用されています。
- Sathish V et al. Cigarette Smoke and Estrogen Signaling in Human Airway Smooth Muscle. Cell Physiol Biochem 36:1101-15 (2015). PubMed: 26113109