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リコンビナントRabMAb

Anti-TMEM43 抗体 [EPR15378(B)] (ab184164)

Submit a review Submit a question References (2)
Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
  • Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
  • Anti-TMEM43 antibody [EPR15378(B)] (ab184164)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15378(B)] to TMEM43
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TMEM43 antibody [EPR15378(B)]
    TMEM43 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15378(B)] to TMEM43

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human spleen and placenta lysates; MDA-MB-435 and Jurkat cell lysates; Human kidney tissue. WB: Mouse brain, heart, kidney and spleen tissues. RAW264.7 and NIH/3T3 whole cell lysates. Rat brain, heart, kidney and spleen tissues. C6 and PC-12 whole cell lysates

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184164の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    May have an important role in maintaining nuclear envelope structure by organizing protein complexes at the inner nuclear membrane. Required for retaining emerin at the inner nuclear membrane.

  • 組織特異性

    Highest expression in placenta. Also found at lower levels in heart, ovary, spleen, small intestine, thymus, prostate and testis.

  • 関連疾患

    Defects in TMEM43 are the cause of familial arrhythmogenic right ventricular dysplasia type 5 (ARVD5) [MIM:604400]; also known as arrhythmogenic right ventricular cardiomyopathy (ARVC5). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.

  • 配列類似性

    Belongs to the TMEM43 family.

  • 細胞内局在

    Endoplasmic reticulum. Nucleus inner membrane. Retained in the inner nuclear membrane through interaction with EMD and A-and B-lamins. The N- and C-termini are orientated towards the nucleoplasm. The majority of the hydrophilic domain resides in the endoplasmic reticulum lumen.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ARVC5 antibody
      • ARVD5 antibody
      • EDMD7 antibody
      • LUMA antibody
      • Protein LUMA antibody
      • Tmem43 antibody
      • TMM43_HUMAN antibody
      • Transmembrane protein 43 antibody
      see all

    画像

    • Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      All lanes : Anti-TMEM43 antibody [EPR15378(B)] (ab184164) at 1/5000 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human placenta tissue lysate
      Lane 3 : MDA-MB-435 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 45 kDa

    • Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Anti-TMEM43 antibody [EPR15378(B)] (ab184164) at 1/20000 dilution + Caco-2 cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 45 kDa

    • Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Western blot - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      All lanes : Anti-TMEM43 antibody [EPR15378(B)] (ab184164) at 1/5000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse heart tissue lysate
      Lane 3 : Mouse kidney tissue lysate
      Lane 4 : Mouse spleen tissue lysate
      Lane 5 : Rat brain tissue lysate
      Lane 6 : Rat heart tissue lysate
      Lane 7 : Rat kidney tissue lysate
      Lane 8 : Rat spleen tissue lysate
      Lane 9 : C6 (rat glial tumor glial cell) whole cell lysate
      Lane 10 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
      Lane 11 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
      Lane 12 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 45 kDa
      Observed band size: 45 kDa


      Exposure time: 180 seconds


      Blocking and diluting buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43  with ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM43 antibody [EPR15378(B)] (ab184164)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TMEM43 with ab184164 at 1/500 dilution. The slide is counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-TMEM43 antibody [EPR15378(B)] (ab184164)
      Anti-TMEM43 antibody [EPR15378(B)] (ab184164)

    参考文献 (2)

    ab184164 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab184164 は 2 報の論文で使用されています。

    • Fenech EJ  et al. Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling. Elife 9:N/A (2020). PubMed: 32614325
    • Xia M  et al. Cell Type-Specific Effects of Mutant DISC1: A Proteomics Study. Mol Neuropsychiatry 2:28-36 (2016). PubMed: 27606318

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