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リコンビナントRabMAb

Anti-TMEM192 抗体 [EPR14330-67] (ab186737)

Submit a review Submit a question References (1)
Western blot - Anti-TMEM192 antibody [EPR14330-67] (ab186737)
  • Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330-67] (ab186737)
  • Anti-TMEM192 antibody [EPR14330-67] (ab186737)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14330-67] to TMEM192
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TMEM192 antibody [EPR14330-67]
    TMEM192 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14330-67] to TMEM192

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    This product was produced with the following immunogens:
    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • U-87 MG, Human fetal liver and Human fetal lung lysates; U-87 MG cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14330-67

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab186737の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Flow Cyt (Intra)
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 組織特異性

    Strongly expressed in kidney, liver, lung and pancreas.

  • 配列類似性

    Belongs to the TMEM192 family.

  • 翻訳後修飾

    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Not N-glycosylated.

  • 細胞内局在

    Lysosome membrane. Late endosome.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ38482 antibody
      • TM192_HUMAN antibody
      • TMEM192 antibody
      • Transmembrane protein 192 antibody

    画像

    • Western blot - Anti-TMEM192 antibody [EPR14330-67] (ab186737)
      Western blot - Anti-TMEM192 antibody [EPR14330-67] (ab186737)
      All lanes : Anti-TMEM192 antibody [EPR14330-67] (ab186737) at 1/10000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal lung lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

      Predicted band size: 31 kDa
      Observed band size: 31 kDa

    • Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330-67] (ab186737)
      Flow Cytometry (Intracellular) - Anti-TMEM192 antibody [EPR14330-67] (ab186737)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedU-87 MG cells labeling TMEM192 with ab186737 at 1/180 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed bygoat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution. 

       

       

    • Anti-TMEM192 antibody [EPR14330-67] (ab186737)
      Anti-TMEM192 antibody [EPR14330-67] (ab186737)

    参考文献 (1)

    ab186737 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab186737 は 1 報の論文で使用されています。

    • Sharma C  et al. Antioxidant functions of DHHC3 suppress anti-cancer drug activities. Cell Mol Life Sci 78:2341-2353 (2021). PubMed: 32986127

    レビューと Q&A

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