Anti-TMEM16A 抗体 [EPR29617-2]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Immunohistochemical analysis of paraffin-embedded Mouse salivary gland tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse salivary gland.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Immunohistochemical analysis of paraffin-embedded Mouse colon cancer tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse colon cancer.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse colon.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining CFTR with ab325171 at a 1 : 2000 (0.259 ug/ml) dilution, ab323181 anti-TMEM16A used at 1 : 1000 (0.532 ug/ml) dilution and ab181547 anti-Insulin used at a 1 : 20000 (0.053 ug/ml) dilution.

Panel A : anti-CFTR (green; Opal™520), anti-TMEM16A (magenta; Opal™690) and anti-Insulin (gray; Opal™570) on mouse pancreas.
Panel B : anti-CFTR staining centroacinar cells in mouse pancreas.
Panel C : anti-TMEM16A staining epithelium in mouse pancreas.
Panel D : anti-Insulin staining islet cells in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325171, ab323181 and ab181547 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling TMEM16A with ab323181 at 1/1000 (0.532 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : Nearly no staining in mouse cardiac muscle.
The section was incubated with ab323181 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : LNCaP(PMID : 29899325), HEK-293(PMID : 27732935)

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (ab323181) at 1/1000 dilution

Lane 1:

Capan-1 (human pancreatic adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, B16-F0

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane1 : 10 seconds, lane2-5 : 26 seconds

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (ab323181) at 1/1000 dilution

Lane 1:

Mouse breast tissue lysate at 20 kDa

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

4T1 (mouse mammary gland carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

B16-F0 (mouse melanoma epithelial-like cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-TMEM16A antibody [EPR29617-2] (AB323181)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse ANO2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TMEM16A antibody [EPR29617-2] (ab323181) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse ANO1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse ANO2 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 110 kDa

false

Exposure time: 10s