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AB325668

Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric)

Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric)

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Chicken Monoclonal tmem119 antibody. Suitable for IHC-P, WB and reacts with Rat, Transfected cell lysate samples.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Representative section of formalin fixed, paraffin-embedded rat brain stem showing microglia cells stained by ab325668 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 400X.

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)
  • WB

Supplier Data

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (AB325668)

Western blotting of mock transfected and TMEM119-FLAG transfected COS-7 lysates with ab325668 (10 µg/mL) and detected with anti-chicken-HRP. The antibody recognizes exogenous TMEM119 only in TMEM119-FLAG transfected COS-7 lysates at 40 kDa.

All lanes:

Western blot - Anti-Tmem119 antibody [L128/43] - Chicken IgY (Chimeric) (ab325668) at 10 µg/mL

Lane 1:

mock transfected COS-7 lysates

Lane 2:

TMEM119-FLAG transfected COS-7 lysates

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 40 kDa

false

Key facts

宿主種

Chicken

クローン性

Monoclonal

クローン番号

L128/43

アイソタイプ

IgY

キャリアフリー

No

交差種

Rat

アプリケーション

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L128/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L128/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information tmem119
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Abcam product promise

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