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リコンビナントRabMAb

Anti-TLR8 抗体 [EPR24499-48] (BSA and Azide free) (ab300430)

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Flow Cytometry (Intracellular) - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)
  • Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)
  • Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)
  • Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24499-48] to TLR8 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free)
    TLR8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR24499-48] to TLR8 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IFmore details
    適用なし: IHC-P,IP or WB

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • ICC/IF: Human PBMC and THP-1 cells. Flow Cyt (Intra): Human peripheral blood mononuclear cells.

  • 特記事項

    ab300430 is a carrier free version of ab300429.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300430の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
特記事項
Flow Cyt (Intra)
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
追加情報
Is unsuitable for IHC-P,IP or WB.

ターゲット情報

  • 機能

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 組織特異性

    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 288 antibody
      • CD288 antibody
      • CD288 antigen antibody
      • MGC119599 antibody
      • MGC119600 antibody
      • TLR 8 antibody
      • Tlr8 antibody
      • TLR8_HUMAN antibody
      • Toll like receptor 8 antibody
      • Toll-like receptor 8 antibody
      see all

    画像

    • Flow Cytometry (Intracellular) - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)
      Flow Cytometry (Intracellular) - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)

      This data was developed using ab300429, the same antibody clone in a different buffer formulation.

      Flow Cytometry (Intracellular) analysis of human peripheral blood mononuclear cells (PBMC) labeling TLR8 with ab300429 at a 1/50 dilution (Right) or Isotype control - Rabbit monoclonal IgG (ab172730) (Left). Cells were fixed with 2% paraformaldehyde and permeabilized with 0.1% Tween-20. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Cells were stained with anti-CD14 conjugated to BV510. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab300429.

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)

      This data was developed using ab300429, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human peripheral blood mononuclear cells) labelling TLR8 with ab300429 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing cytoplasmic and membrane staining in some human PBMCs. The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)
      Immunocytochemistry/ Immunofluorescence - Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (ab300430)

      This data was developed using ab300429, the same antibody clone in a different buffer formulation.

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia monocytes) labelling TLR8 with ab300429 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing weak staining in THP-1 cells treated 12-O-Tetradecanoylphorbol-13-acetate (80 nM) for 16 h, and showing increased staining at the membrane in THP-1 cell treated with 12-O-Tetradecanoylphorbol-13-acetate (80nM) for 16 h, then together with human Interferon-γ (20ng/ml) for another 8 h. The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.

    • Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)
      Anti-TLR8 antibody [EPR24499-48] (BSA and Azide free) (AB300430)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300430 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300430 は論文での使用が確認できていません。

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