Anti-TLR8 抗体 [EPR24499-48] (ab300429)

Flow Cytometry (Intracellular) analysis of human peripheral blood mononuclear cells (PBMC) labeling TLR8 with ab300429 at a 1/50 dilution (Right) or Isotype control - Rabbit monoclonal IgG (ab172730) (Left). Cells were fixed with 2% paraformaldehyde and permeabilized with 0.1% Tween-20. A Goat Anti-Rabbit IgG (Alexa Fluor^® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Cells were stained with anti-CD14 conjugated to BV510. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab300429.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human peripheral blood mononuclear cells) labelling TLR8 with ab300429 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing cytoplasmic and membrane staining in some human PBMCs. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) at 1/200 dilution.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia monocytes) labelling TLR8 with ab300429 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (green). Confocal image showing weak staining in THP-1 cells treated 12-O-Tetradecanoylphorbol-13-acetate (80 nM) for 16 h, and showing increased staining at the membrane in THP-1 cell treated with 12-O-Tetradecanoylphorbol-13-acetate (80nM) for 16 h, then together with human Interferon-γ (20ng/ml) for another 8 h. The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) (red) at 1/200 dilution.