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Anti-TLR8 抗体 [44C143] (ab85859)

Submit a review Submit a question References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [44C143] (ab85859)
  • Flow Cytometry (Intracellular) - Anti-TLR8 antibody [44C143] (ab85859)
  • Western blot - Anti-TLR8 antibody [44C143] (ab85859)

Key features and details

  • Mouse monoclonal [44C143] to TLR8
  • Suitable for: WB, IHC-P, Flow Cyt (Intra)
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

PE

製品の概要

  • 製品名

    Anti-TLR8 antibody [44C143]
    TLR8 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [44C143] to TLR8

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human TLR8 aa 750-850 conjugated to keyhole limpet haemocyanin.
    Database link: Q9NR97

  • ポジティブ･コントロール

    • IHC-P: Human spleen tissue. Flow Cyt (Intra): Ramos cells. WB: 293 transfected with human TLR8.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab85859の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 120 kDa.
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

特記事項
WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 120 kDa.
IHC-P
Use a concentration of 5 µg/ml.
Flow Cyt (Intra)
Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 組織特異性

    Detected in brain, heart, lung, liver, placenta, in monocytes, and at lower levels in CD11c+ immature dendritic cells.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 23 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 288 antibody
      • CD288 antibody
      • CD288 antigen antibody
      • MGC119599 antibody
      • MGC119600 antibody
      • TLR 8 antibody
      • Tlr8 antibody
      • TLR8_HUMAN antibody
      • Toll like receptor 8 antibody
      • Toll-like receptor 8 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [44C143] (ab85859)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR8 antibody [44C143] (ab85859)

      ab85859 at 5 µg/ml staining TLR8 in formalin fixed and paraffin embedded human spleen tissue

    • Flow Cytometry (Intracellular) - Anti-TLR8 antibody [44C143] (ab85859)
      Flow Cytometry (Intracellular) - Anti-TLR8 antibody [44C143] (ab85859)

      ab85859 at 0.5µg in 1x106 Ramos cells Shaded histogram represents Ramos cells without antibody; green represents isotype control; purple represents TLR8 antibody.

       

    • Western blot - Anti-TLR8 antibody [44C143] (ab85859)
      Western blot - Anti-TLR8 antibody [44C143] (ab85859)
      Anti-TLR8 antibody [44C143] (ab85859) at 2 µg/ml + 293 cell lysate transfected with Human TLR8

      Predicted band size: 120 kDa
      Observed band size: 120 kDa

    参考文献 (3)

    ab85859 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab85859 は 3 報の論文で使用されています。

    • Ahmad R  et al. Increased adipose tissue expression of TLR8 in obese individuals with or without type-2 diabetes: significance in metabolic inflammation. J Inflamm (Lond) 13:38 (2016). PubMed: 27980459
    • Li Z  et al. Inhibition of IRAK1/4 sensitizes T cell acute lymphoblastic leukemia to chemotherapies. J Clin Invest 125:1081-97 (2015). PubMed: 25642772
    • Luangsay S  et al. Expression and functionality of Toll- and RIG-like receptors in HepaRG cells. J Hepatol 63:1077-85 (2015). PubMed: 26144659

    レビューと Q&A

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