Anti-TLR6 抗体 (ab37072)
Key features and details
- Rabbit polyclonal to TLR6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TLR6 antibody
TLR6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TLR6 -
由来種
Rabbit -
特異性
TLR6 antibody is specific to TLR6. It does not recognize TLR1. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to 15 amino acids near the amino terminus of the human TLR6 protein.
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ポジティブ･コントロール
- Jurkat whole cell lysate (ab7899).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This antibody was purified by affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37072の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa).
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 92 kDa). |
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ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Participates in the innate immune response to Gram-positive bacteria and fungi. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR2. -
組織特異性
Detected in monocytes, CD11c+ immature dendritic cells, plasmacytoid pre-dendritic cells and dermal microvessel endothelial cells. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 12 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 TIR domain. -
細胞内局在
Cell membrane. Cytoplasmic vesicle > phagosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10333 Human
- Omim: 605403 Human
- SwissProt: Q9Y2C9 Human
- Unigene: 575090 Human
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別名
- CD286 antibody
- CD286 antigen antibody
- TLR 6 antibody
see all
画像
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Western blot - Anti-TLR6 antibody (ab37072)Western Blot of Daudi cell lysate labeling TLR6 with Anti-TLR6 antibody (ab37072) at 1µg/ml in (1) the absence and (2) the presence of blocking peptide..
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Western blot - Anti-TLR6 antibody (ab37072)Lane 1 : Anti-TLR6 antibody (ab37072) at 0.5 µg/ml
Lane 2 : Anti-TLR6 antibody (ab37072) at 1 µg/ml
Lane 3 : Anti-TLR6 antibody (ab37072) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/10000 dilution
Predicted band size: 92 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 80 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-TLR6 antibody (ab37072)ab37072 at 10 µg/ml staining TLR6 in Jurkat cells by ICC/IF
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab37072 は 7 報の論文で使用されています。
- Lei L et al. Comprehensive Diagnostics of Diabetic Nephropathy by Transcriptome RNA Sequencing. Diabetes Metab Syndr Obes 15:3069-3080 (2022). PubMed: 36237968
- Al-Sadi R et al. Bifidobacterium bifidum Enhances the Intestinal Epithelial Tight Junction Barrier and Protects against Intestinal Inflammation by Targeting the Toll-like Receptor-2 Pathway in an NF-κB-Independent Manner. Int J Mol Sci 22:N/A (2021). PubMed: 34360835
- Galoian K et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803
- Moretti IF et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). PubMed: 29912993
- Salyer ACD & David SA Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists. Hum Vaccin Immunother 14:1686-1696 (2018). PubMed: 29852079
- Ma L et al. Developmental expression of Toll-like receptors in the guinea pig lung. Mol Med Rep 15:1243-1251 (2017). PubMed: 28098883
- Choteau L et al. Role of TLR1, TLR2 and TLR6 in the modulation of intestinal inflammation and Candida albicans elimination. Gut Pathog 9:9 (2017). PubMed: 28289440