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AB13876

Anti-TLR5 抗体 [19D759.2]

Anti-TLR5 antibody [19D759.2]

4

(11 Reviews)

|

(17 Publications)

Mouse Monoclonal TLR5 antibody. Suitable for ICC, IP, WB, IHC-P, ICC/IF, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 17 publications. Immunogen corresponding to Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

TIL3, TLR5, Toll-like receptor 5, Toll/interleukin-1 receptor-like protein 3

2 Images
Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (AB13876)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TLR5 antibody [19D759.2] (AB13876)

Flow Cytometry (Intracellular) analysis of TLR5 in 106 Ramos cells using (A) 2μg of isotype control and (B) 2μg of ab13876

Western blot - Anti-TLR5 antibody [19D759.2] (AB13876)
  • WB

Unknown

Western blot - Anti-TLR5 antibody [19D759.2] (AB13876)

Western blot analysis of TLR5 in human Ramos cell lysate (A) and mouse Raw cell,lysate (B) using ab13876 at 2μg/ml

All lanes:

Western blot - Anti-TLR5 antibody [19D759.2] (ab13876) at 2 µg/mL

Lane 1:

Ramos cell lysate

Lane 2:

Raw cell lysate

Predicted band size: 97 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

19D759.2

アイソタイプ

IgG2a

軽鎖のタイプ

kappa

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

ICC, ICC/IF, Flow Cyt (Intra), IP, IHC-Fr, WB, IHC-P

applications

免疫原

Synthetic Peptide within Human TLR5 aa 700-800 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O60602

特異性

This antibody recognizes an intracellular epitope in the cytoplasmic domain of TLR5.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer pH 6.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>(see Miller et al).</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg/cells for 6.00000 µg/cells", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody</p>" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval with citrate buffer pH 6.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p>(see Miller et al).</p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 5 (TLR5) also known as CD285 is a protein that functions mechanically as a pattern recognition receptor. It has a mass of about 98 kDa and is expressed mainly in the tissues of the lung intestine and urogenital tract. TLR5 recognizes pathogen-associated molecular patterns specifically identifying and binding to flagellin a protein component of bacterial flagella. This engagement initiates a signaling cascade that results in an immune response.
Biological function summary

This receptor plays a central role in the innate immune system. TLR5 is part of a complex network of receptors that detect microbial infections and activate immune defense. When TLR5 binds to flagellin it triggers the production of inflammatory cytokines and activation of adaptive immune responses. This interaction helps in mobilizing immune cells to the site of infection providing the body with a robust defense mechanism against bacterial pathogens.

Pathways

TLR5 activation is an important component of the NF-kB signaling and MAPK pathways. These pathways lead to the transcription of genes involved in inflammation and immune response. TLR5 interacts with MyD88 an adaptor protein to facilitate the downstream signaling cascade. This involvement links TLR5 to not only NF-kB pathway but also connects it to other Toll-like receptors further integrating the immune signaling network.

TLR5 has been connected to inflammatory bowel disease and chronic obstructive pulmonary disease (COPD). Abnormal TLR5 signaling can lead to excessive inflammation contributing to these conditions. In COPD TLR5 and its associated cytokines can exacerbate lung inflammation while in inflammatory bowel disease dysregulation may trigger improper intestinal immune responses. Research into TLR5's role in these diseases can illuminate potential therapeutic targets.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response (PubMed : 11323673, PubMed : 18490781). Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota (PubMed : 29934223). Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response (PubMed : 11489966, PubMed : 20855887). Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life (By similarity).
See full target information TLR5
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文献 (17)

Recent publications for all applications. Explore the full list and refine your search

Gut 72:2112-2122 PubMed37491158

2023

generated butyrate boosts anti-PD-1 efficacy in colorectal cancer by activating cytotoxic CD8 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Kang,Changan Liu,Yanqiang Ding,Yunbi Ni,Fenfen Ji,Harry Cheuk Hay Lau,Lanping Jiang,Joseph Jy Sung,Sunny H Wong,Jun Yu

BMJ open gastroenterology 10: PubMed37433685

2023

Toll-like receptor 5 tunes hepatic and pancreatic stellate cells activation.

Applications

Unspecified application

Species

Unspecified reactive species

Pietro Di Fazio,Sophia Mielke,Isabell T Böhm,Malte Buchholz,Sami Matrood,Detlef Schuppan,Thaddeus Wissniowski

Phytotherapy research : PTR 37:731-742 PubMed36196887

2022

Effect of curcumin on regulatory B cells in chronic colitis mice involving TLR/MyD88 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Huang,Tiantian Wu,Youbao Zhong,Jiaqi Huang,Zengping Kang,Bugao Zhou,Haimei Zhao,Duanyong Liu

Nutrients 14: PubMed35893896

2022

and Its Metabolite Butyrate Inhibit Colitis and Upregulate TLR5 through the SP3 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangcong Ruan,Minjia Chen,Lu Chen,Fenghua Xu,Zhifeng Xiao,Ailin Yi,Yuting Tian,Yi Ping,Linling Lv,Yi Cheng,Yanling Wei

Cell death & disease 13:183 PubMed35210436

2022

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huaili Jiang,Lei Zhou,Na Shen,Xianhui Ning,Daquan Wu,Kanglun Jiang,Xinsheng Huang

Acta neuropathologica communications 8:159 PubMed32912327

2020

Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Masataka Ifuku,Lukas Hinkelmann,Leonard D Kuhrt,Ibrahim E Efe,Victor Kumbol,Alice Buonfiglioli,Christina Krüger,Philipp Jordan,Marcus Fulde,Mami Noda,Helmut Kettenmann,Seija Lehnardt

Tissue barriers 8:1728165 PubMed32079482

2020

Short-term tissue permeability actions of dextran sulfate sodium studied in a colon organ culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Elisabeth M Danielsen,Alba De Haro Hernando,Mohammad Yassin,Karina Rasmussen,Jørgen Olsen,Gert H Hansen,E Michael Danielsen

Journal of veterinary science 20:e70 PubMed31775197

2019

Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Ho Oh,Young Saeng Kim Cho,Ho Bin Lee,Sang Mok Lee,Whee Soo Kim,Liang Hong,Chong Su Cho,Yun Jaie Choi,Sang Kee Kang

Leukemia & lymphoma 60:2646-2657 PubMed31017033

2019

The prognostic value of toll-like receptor5 and programmed cell death-ligand1 in patients with peripheral T-cell non-Hodgkin lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengqi Sun,Wenjia Su,Jingrong Qian,Hongxue Meng,Hongfei Ji,Yupeng Liu,Yue Zhang,Wenhui Li,Qingyuan Zhang,Shu Zhao

Sisli Etfal Hastanesi tip bulteni 52:196-200 PubMed32595398

2018

Evaluation of Toll-Like Receptor Expression with Clinicopathologic Variables in Endometrium Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Berat Berrin Gençoğlu Bakbak,Tolgay Tuyan Ilhan,Aybike Pekin,Özlem Seçilmiş Kerimoğlu,Setenay Arzu Yılmaz,Ayşegül Kebapçılar,Nasuh Utku Doğan,Pınar Karabağlı,Çetin Çelik
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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