Anti-TLR3 抗体 [40C1285] (ab13915)
Key features and details
- Mouse monoclonal [40C1285] to TLR3
- Suitable for: IHC-P, Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-TLR3 antibody [40C1285]
TLR3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [40C1285] to TLR3 -
由来種
Mouse -
特異性
Negative results in WB have been obtained for mouse interstine lysates (human intestine is a positive control). The protein is heavily glycosylated and the observed band size should be higher than the predicted 103kDa. The protein is found at different molecular weight in different tissues so optimization may be necessary. -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra), WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human TLR3 aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: O15455 -
ポジティブ・コントロール
- Human intestine tissue lysate, mouse spleen Flow Cyt (Intra): human B cells, human monocytes, SW480, SW620 (colon cancer)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
40C1285 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13915の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended.
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Flow Cyt (Intra) |
Use 2-5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes.
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特記事項 |
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IHC-P
Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended. |
Flow Cyt (Intra)
Use 2-5µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes. |
ターゲット情報
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機能
Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. -
組織特異性
Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons. -
関連疾患
Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system. -
配列類似性
Belongs to the Toll-like receptor family.
Contains 22 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
Contains 1 TIR domain. -
ドメイン
ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18. -
翻訳後修飾
Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding. -
細胞内局在
Endoplasmic reticulum membrane. Endosome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7098 Human
- Entrez Gene: 142980 Mouse
- Omim: 603029 Human
- SwissProt: O15455 Human
- SwissProt: Q99MB1 Mouse
- Unigene: 657724 Human
- Unigene: 33874 Mouse
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別名
- CD283 antibody
- CD283 antigen antibody
- IIAE2 antibody
see all
画像
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Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse intestine using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. The image shows a punctate staining of the ER and endosomes in a subset of cells in Peyer's patches (organized lymphoid nodules) in the tested section.
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All lanes : Anti-TLR3 antibody [40C1285] (ab13915) at 3 µg/ml
Lane 1 : Untransfected HEK293 cell lysate
Lane 2 : HEK293 cell lysate transfected with human TLR3 cDNA whole cell lysate
Lane 3 : Human intestine tissue lysate
Lane 4 : Human placenta tissue lysate
Lane 5 : Human heart tissue lysate
Lane 6 : Human ovary tissue lysate
Secondary
All lanes : Goat anti-mouse HRP conjugate
Predicted band size: 108 kDa -
Flow cytometry (Intracellular) analysis of human B cells using ab13915 at 1µg/106 cells followed by FITC conjugated secondary antibody (Red). Green shows isotype control.
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Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20
Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).µ g/lane human intestine tissue lysate (lane C). -
Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse colon using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was found in subset of cells at the bases of the crypts.
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ab13915 at 2µg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cyt (Intra). A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915.
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ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.
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ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 µg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black l
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (25)
ab13915 は 25 報の論文で使用されています。
- Tucker MH et al. IRF7 and UNC93B1 variants in an infant with recurrent herpes simplex virus infection. J Clin Invest 133:N/A (2023). PubMed: 37097753
- Fan Y et al. Pinitol attenuates LPS-induced pneumonia in experimental animals: Possible role via inhibition of the TLR-4 and NF-κB/IκBα signaling cascade pathway. J Biochem Mol Toxicol 35:e22622 (2021). PubMed: 32926510
- Mikhalkevich N et al. Response of human macrophages to gamma radiation is mediated via expression of endogenous retroviruses. PLoS Pathog 17:e1009305 (2021). PubMed: 33556144
- Bhagat S et al. Hypoxia induced up-regulation of tissue factor is mediated through extracellular RNA activated Toll-like receptor 3-activated protein 1 signalling. Blood Cells Mol Dis 84:102459 (2020). PubMed: 32559654
- Lomphithak T et al. Receptor-interacting protein kinase 1 is a key mediator in TLR3 ligand and Smac mimetic-induced cell death and suppresses TLR3 ligand-promoted invasion in cholangiocarcinoma. Cell Commun Signal 18:161 (2020). PubMed: 33036630