Anti-TLR3 抗体 [40C1285]

• Flow Cyt (Intra)

PubMed

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (AB13915)

ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 μg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black l

This image is a courtesy of Tanja Matijevic

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (AB13915)

Flow cytometry (Intracellular) analysis of human B cells using ab13915 at 1μg/10⁶ cells followed by FITC conjugated secondary antibody (Red). Green shows isotype control.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TLR3 antibody [40C1285] (AB13915)

ab13915 at 2μg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cyt (Intra). A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (AB13915)

Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse colon using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was found in subset of cells at the bases of the crypts.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (AB13915)

Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse intestine using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. The image shows a punctate staining of the ER and endosomes in a subset of cells in Peyer's patches (organized lymphoid nodules) in the tested section.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLR3 antibody [40C1285] (AB13915)

ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.

• WB

Unknown

Western blot - Anti-TLR3 antibody [40C1285] (AB13915)

Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 μg/lane human intestine tissue lysate (lane C).

Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).

All lanes:

Western blot - Anti-TLR3 antibody [40C1285] (ab13915)

Predicted band size: 104 kDa

false

• WB

Supplier Data

Western blot - Anti-TLR3 antibody [40C1285] (AB13915)

All lanes:

Western blot - Anti-TLR3 antibody [40C1285] (ab13915) at 3 µg/mL

Lane 1:

Untransfected HEK293 cell lysate

Lane 2:

HEK293 cell lysate transfected with human TLR3 cDNA whole cell lysate

Lane 3:

Human intestine tissue lysate

Lane 4:

Human placenta tissue lysate

Lane 5:

Human heart tissue lysate

Lane 6:

Human ovary tissue lysate

Secondary

All lanes:

Goat anti-mouse HRP conjugate

Predicted band size: 104 kDa

false

• WB

CiteAb

Western blot - Anti-TLR3 antibody [40C1285] (AB13915)

TLR3 western blot using anti-TLR3 antibody [40C1285] ab13915. Publication image and figure legend from Li, C., Huang, J., et al., 2019, Stem Cells Int, PubMed 31885603.

ab13915 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab13915 please see the product overview.

Western blot detection of immune-related proteins TLRs, IDO, and NF-κB stimulated by different PLP concentrations. TLR3, TLR4, NF-κB, and IDO had the highest protein expression levels, while TLR6 protein expression was the lowest at 50 ng/mL PLP.

false