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Anti-TLR2 抗体 [6C2] (ab11864)

Reviews (1)Q&A (1)References (4)

Key features and details

  • Rat monoclonal [6C2] to TLR2
  • Suitable for: IP, ICC/IF, Flow Cyt
  • Reacts with: Mouse
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-TLR2 antibody [6C2]
    TLR2 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [6C2] to TLR2

  • 由来種

    Rat

  • アプリケーション

    適用あり: IP, ICC/IF, Flow Cytmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse

  • 免疫原

    CHO cells expressing mouse TLR2.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab11864の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
特記事項
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
追加情報
Is unsuitable for WB.

ターゲット情報

  • 機能

    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.

  • 組織特異性

    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.

  • 翻訳後修飾

    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD282 antibody
      • CD282 antigen antibody
      • TIL 4 antibody
      • TIL4 antibody
      • TLR 2 antibody
      • TLR2 antibody
      • TLR2_HUMAN antibody
      • Toll like receptor 2 antibody
      • Toll like receptor 2 precursor antibody
      • Toll-like receptor 2 antibody
      • Toll/interleukin 1 receptor like 4 antibody
      • Toll/interleukin 1 receptor like protein 4 antibody
      • Toll/interleukin receptor like protein 4 antibody
      • Toll/interleukin-1 receptor-like protein 4 antibody
      see all

    参考文献 (4)

    ab11864 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab11864 は 4 報の論文で使用されています。

    • Xu X  et al. Bone Marrow Stromal Cell Antigen 2: Is a Potential Neuroinflammation Biomarker of SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis in Pre-symptomatic Stage. Front Neurosci 15:788730 (2021). PubMed: 35197819
    • Yang Q  et al. CD157 Confers Host Resistance to Mycobacterium tuberculosis via TLR2-CD157-PKCzeta-Induced Reactive Oxygen Species Production. mBio 10:N/A (2019). PubMed: 31455656
    • Wang M  et al. Microbial hijacking of complement-toll-like receptor crosstalk. Sci Signal 3:ra11 (2010). PubMed: 20159852
    • Nilsen N  et al. Lipopolysaccharide and double-stranded RNA up-regulate toll-like receptor 2 independently of myeloid differentiation factor 88. J Biol Chem 279:39727-35 (2004). PubMed: 15190057

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-TLR2 antibody [6C2]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Primary macrophage)
    Specification
    Primary macrophage
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Acetone, 5 min, -20
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Mar 26 2013

    Question

    I need an information about your product no. ab11864 (TLR2 antibody[6C2]). I will investigate bacterial proteins whether they can activate TLR2 and cytokines as end-products can be secreted using mouse macrophage cells. However, application notes on your antibody TLR2 antibody [6C2] do not describe our objectives, that is, blocking property is not included. Therefore, I want to know whether it can block mouse TLR2 for blocking experiments. Can it be used as an antagonist or a blocker for mouse TLR2 ?

    Read More
    Answer

    Thank you for your enquiry. All the information that we have regarding this antibody is located on the online datasheet. To our knowledge, ab11864 has only been tested for application in Immunoprecipitation, Immunofluorescence, and Flow Cytometry / FACS and has been found to be unsuitable for Western blotting. At this time, we don't have any information about whether it can be used in blocking experiments. If you have any more questions, please contact us again.

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