AB64833

Anti-TLE4 抗体

Name: Anti-TLE4 抗体 (ab64833) | Abcam
Brand: Abcam Limited
SKU: AB64833
Price: 74000 JPY
Availability: InStock
Rating: 2 (2 reviews)

Anti-TLE4 antibody

(2 Reviews)

(7 Publications)

Rabbit Polyclonal TLE4 antibody. Suitable for WB and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TLE4.

別名を表示する

GRG4, KIAA1261, TLE4, Transducin-like enhancer protein 4, Grg-4, Groucho-related protein 4

2 Images

Supplier Data

Western blot - Anti-TLE4 antibody (AB64833)

All lanes:

Western blot - Anti-TLE4 antibody (ab64833) at 1/500 dilution

Lane 1:

Jurkat cell extract at 30 µg

Lane 2:

293 cell extract at 30 µg

Lane 3:

HuvEc cell extract at 30 µg

Predicted band size: 84 kDa

Observed band size: 84 kDa

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Supplier Data

Western blot - Anti-TLE4 antibody (AB64833)

All lanes:

Western blot - Anti-TLE4 antibody (ab64833) at 1/1000 dilution

All lanes:

Rat muscle lysate

Predicted band size: 84 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Rat, Human

アプリケーション

applications

免疫原

Synthetic Peptide within Human TLE4. The exact immunogen used to generate this antibody is proprietary information.

Q04727

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Transducin-like enhancer of split 4 (TLE4) is a transcriptional corepressor involved in the regulation of gene expression. It is also known as ESG (Enhancer of split groucho) or GRG4. TLE4 belongs to the Groucho/TLE protein family which shares a general structure characterized by WD40 repeats. The protein has a molecular mass of about 82 kDa. TLE4 expresses in several tissues including the brain heart and peripheral blood leukocytes indicating its broad functional importance.

Biological function summary

TLE4 participates in the repression of transcription by forming a multiprotein complex. It acts as a corepressor that interacts with various transcription factors often modulating the expression of genes involved in developmental processes. TLE4 commonly partners with transcription factors from the basic helix-loop-helix (bHLH) family which play a significant role in the regulation of neuronal and muscular development. The repression activity of TLE4 makes it a temporal regulator helping maintain and coordinate gene expression during cellular differentiation.

Pathways

TLE4 functions within the Wnt signaling and Notch signaling pathways. Both of these pathways are important for cell fate determination and organ development. In the Wnt pathway TLE4 interacts with beta-catenin to modulate transcriptional activity contributing to cellular responses to external signals. In the Notch signaling pathway TLE4 associates with core transcription factors like CSL (CBF1/Suppressor of Hairless/Lag1) to regulate the expression of Notch target genes facilitating diverse developmental processes.

TLE4 has links to hematological malignancies and neurological conditions. Its dysregulation can contribute to conditions like acute myeloid leukemia (AML) alongside proteins such as RUNX1 which play a role in hematopoiesis and myeloid lineage development. Additionally altered TLE4 expression has associations with neurodevelopmental disorders as its interaction with key proteins in neuronal differentiation is disturbed affecting neuronal function and development.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression (By similarity). Involved in transcriptional repression of GNRHR and enhances MSX1-mediated transcriptional repression of CGA/alpha-GSU (By similarity).

See full target information TLE4

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:8043 PubMed39271675

2024

Conserved transcriptional regulation by BRN1 and BRN2 in neocortical progenitors drives mammalian neural specification and neocortical expansion.

Applications

Unspecified application

Species

Unspecified reactive species

Soraia Barão,Yijun Xu,José P Llongueras,Rachel Vistein,Loyal Goff,Kristina J Nielsen,Byoung-Il Bae,Richard S Smith,Christopher A Walsh,Genevieve Stein-O'Brien,Ulrich Müller

Cancer discovery 14:2430-2449 PubMed39121480

2024

Epigenetic and Oncogenic Inhibitors Cooperatively Drive Differentiation and Kill KRAS-Mutant Colorectal Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Loi,Amy E Schade,Carrie L Rodriguez,Anjana Krishnan,Naiara Perurena,Van T M Nguyen,Yilin Xu,Marina Watanabe,Rachel A Davis,Alycia Gardner,Natalie F Pilla,Kaia Mattioli,Olesja Popow,Nuray Gunduz,Tamsin R M Lannagan,Samantha Fitzgerald,Ewa T Sicinska,Jia-Ren Lin,William Tan,Lauren K Brais,Kevin M Haigis,Marios Giannakis,Kimmie Ng,Sandro Santagata,Kristian Helin,Owen J Sansom,Karen Cichowski

Journal of Cancer 15:4759-4776 PubMed39006072

2024

TLE4 downregulation identified by WGCNA and machine learning algorithm promotes papillary thyroid carcinoma progression via activating JAK/STAT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Lin,Beichen Cai,Qian Lin,Xinjian Lin,Biao Wang,Xiangjin Chen

Nature communications 11:1313 PubMed32152318

2020

Functional hypoxia drives neuroplasticity and neurogenesis via brain erythropoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Debia Wakhloo,Franziska Scharkowski,Yasmina Curto,Umer Javed Butt,Vikas Bansal,Agnes A Steixner-Kumar,Liane Wüstefeld,Ashish Rajput,Sahab Arinrad,Matthias R Zillmann,Anna Seelbach,Imam Hassouna,Katharina Schneider,Abdul Qadir Ibrahim,Hauke B Werner,Henrik Martens,Kamilla Miskowiak,Sonja M Wojcik,Stefan Bonn,Juan Nacher,Klaus-Armin Nave,Hannelore Ehrenreich

PloS one 13:e0191689 PubMed29370269

2018

The histone demethylase LSD1 regulates inner ear progenitor differentiation through interactions with Pax2 and the NuRD repressor complex.

Applications

Unspecified application

Species

Unspecified reactive species

Dharmeshkumar Patel,Atsushi Shimomura,Sreeparna Majumdar,Matthew C Holley,Eri Hashino

PloS one 9:e105557 PubMed25153823

2014

The corepressor Tle4 is a novel regulator of murine hematopoiesis and bone development.

Applications

Species

Mouse

Justin C Wheat,Daniela S Krause,Thomas H Shin,Xi Chen,Jianfeng Wang,Dacheng Ding,Rae'e Yamin,David A Sweetser

Molecular and cellular biology 34:233-45 PubMed24190972

2013

Tle4 regulates epigenetic silencing of gamma interferon expression during effector T helper cell tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Sanmay Bandyopadhyay,Rut Valdor,Fernando Macian

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-TLE4 抗体

Anti-TLE4 antibody

Western blot - Anti-TLE4 antibody (AB64833)

Western blot - Anti-TLE4 antibody (AB64833)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (7)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise