Anti-TLE3/ESG 抗体 (ab185508)
Key features and details
- Rabbit polyclonal to TLE3/ESG
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TLE3/ESG antibody
TLE3/ESG 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TLE3/ESG -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment corresponding to Human TLE3/ESG aa 150-250.
Database link: Q04726 -
ポジティブ･コントロール
- Human vulva/anal skin tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185508の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 83 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| IHC-P | (1) |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 83 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. -
組織特異性
Placenta and lung. -
配列類似性
Belongs to the WD repeat Groucho/TLE family.
Contains 7 WD repeats. -
翻訳後修飾
Ubiquitinated by XIAP/BIRC4. This ubiquitination does not affect its stability, nuclear localization, or capacity to tetramerize but inhibits its interaction with TCF7L2/TCF4. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7090 Human
- Entrez Gene: 21887 Mouse
- Entrez Gene: 84424 Rat
- Omim: 600190 Human
- SwissProt: Q04726 Human
- SwissProt: Q08122 Mouse
- SwissProt: Q9JIT3 Rat
- Unigene: 287362 Human
see all -
別名
- E(sp1) homolog Drosophila antibody
- Enhancer of split groucho 3 antibody
- Enhancer of split groucho-like protein 3 antibody
see all
画像
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Western blot - Anti-TLE3/ESG antibody (ab185508)Anti-TLE3/ESG antibody (ab185508) + HeLa nuclear extract
Predicted band size: 83 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody (ab185508)Immunohistochemical analysis of paraffin-embedded Human vulva/anal skin tissue, labeling TLE3/ESG using ab185508 at a 1/500 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody (ab185508)Immunofluorescent analysis of HeLa cells labelling TLE3/ESG with ab185508. Antibody staining is shown in green.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab185508 は論文での使用が確認できていません。