JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213596

Anti-TLE3/ESG 抗体 [CL3573]

Anti-TLE3/ESG antibody [CL3573]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal TLE3/ESG antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TLE3 aa 150-250.

別名を表示する

KIAA1547, TLE3, Transducin-like enhancer protein 3, Enhancer of split groucho-like protein 3, ESG3

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human skeletal muscle tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody [CL3573] (AB213596)

PFA-fixed, Triton X-100 permeabilized HeLa cells stained for TLE3 (Green) using ab213596 at 4 μg/ml in ICC/IF.

Microtublues are stained red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human small intestine tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human kidney tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human oral mucosa tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • WB

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HEK293 cell lysate

Predicted band size: 83 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human cervix tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TLE3/ESG antibody [CL3573] (AB213596)

Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labeling TLE3/ESG with ab213596 at 1/10000 dilution.

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)
  • WB

Supplier Data

Western blot - Anti-TLE3/ESG antibody [CL3573] (AB213596)

All lanes:

Western blot - Anti-TLE3/ESG antibody [CL3573] (ab213596) at 1/500 dilution

All lanes:

HeLa nuclear lysate

Predicted band size: 83 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CL3573

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human TLE3 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q04726

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10000 - 1/20000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TLE3 also known as ESG is a transcriptional corepressor with a molecular mass of around 80 kDa. It belongs to the transducin-like enhancer of split (TLE) family which interacts with DNA-binding proteins to modulate gene transcription. TLE3 is expressed mainly in the nucleus of cells and has notable expression in various tissues including the brain skeletal muscle and adipose tissues. Its mechanical role involves binding to nuclear receptors and influencing the transcriptional repression process by preventing the assembly of transcriptional activators on target genes.
Biological function summary

TLE3 serves as a critical modulator of differentiation pathways. It often functions as part of a broader repressor complex working closely with Groucho-related proteins. These complexes exert influence over cell lineage commitment and differentiation particularly in the context of adipogenesis and muscle development. TLE3 helps direct stem cell fate by repressing pro-differentiation signals maintaining cells in a less differentiated state until specific developmental cues trigger further differentiation.

Pathways

The function of TLE3 intersects with Wnt and Notch signaling pathways both essential for cellular growth and differentiation processes. TLE3 associates with proteins like TCF/LEF during Wnt signaling to suppress target gene activation therefore regulating cellular proliferation and differentiation. In the Notch pathway TLE3 interacts with the CSL transcription factor contributing to the repression of Notch-responsive genes which play a part in the maintenance of stem cell populations and tissue homeostasis.

TLE3 shows a connection with cancer and metabolic syndromes. In cancer aberrant expression of TLE3 can lead to disrupted cell differentiation promoting oncogenesis. For example its interaction with TCF/LEF proteins in the Wnt pathway can affect tumor suppressor gene expression. In metabolic disorders TLE3 impacts adipogenesis contributing to obesity-linked insulin resistance. Dysregulation in these pathways linked to proteins like PPARγ highlights TLE3's role in disease pathophysiology and potential as a therapeutic target.
style
left-column
style
left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

ターゲットの情報

Transcriptional corepressor that binds to a number of transcription factors (PubMed : 28689657). Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling (PubMed : 28689657). The effects of full-length TLE family members may be modulated by association with dominant-negative AES (By similarity).
See full target information TLE3
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Genes & development 33:747-762 PubMed31123067

2019

Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Pearson,Anne Loft,Prashant Rajbhandari,Judith Simcox,Sanghoon Lee,Peter Tontonoz,Susanne Mandrup,Claudio J Villanueva

The Journal of biological chemistry 292:12885-12894 PubMed28607151

2017

The transcriptional co-repressor TLE3 regulates myogenic differentiation by repressing the activity of the MyoD transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

Shoichiro Kokabu,Chihiro Nakatomi,Takuma Matsubara,Yusuke Ono,William N Addison,Jonathan W Lowery,Mariko Urata,Aaron M Hudnall,Suzuro Hitomi,Mitsushiro Nakatomi,Tsuyoshi Sato,Kenji Osawa,Tetsuya Yoda,Vicki Rosen,Eijiro Jimi
View all publications
style
left-column
style
left-column
style
right-column

代替製品

Primary Antibodies

AB185508

Anti-TLE3/ESG antibody

Immunocytochemistry/ Immunofluorescence - Anti-TLE3/ESG antibody (AB185508)

  • 4
  • 1

組合せ製品

Primary Antibodies

AB183742

Anti-TLE 1 antibody [EPR9386(2)]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-TLE 1 antibody [EPR9386(2)] (AB183742)

  • 5
  • 1
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (AB52866)

  • 5
  • 31
Primary Antibodies

AB317817

Anti-SRRM2 antibody [EPR28620-54]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Flow Cytometry (Intracellular) - Anti-SRRM2 antibody [EPR28620-54] (AB317817)

  • 5
  • 1

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com