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Anti-Tissue Plasminogen Activator 抗体 [H27B6] (ab28374)

Submit a review Q&A (3)References (4)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPA Tissue Plasminogen Activator antibody [H27B6] (ab28374)

    Key features and details

    • Mouse monoclonal [H27B6] to Tissue Plasminogen Activator
    • Suitable for: WB, ELISA, IHC-P
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (ab157469)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-Tissue Plasminogen Activator antibody [H27B6]
      Tissue Plasminogen Activator 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [H27B6] to Tissue Plasminogen Activator

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WB, ELISA, IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat

    • 免疫原

      Recombinant full length protein (Mouse)

    • エピトープ

      protease domain (see Declerck et al)

    • ポジティブ･コントロール

      • This antibody gave a positive result in IHC in the following FFPE tissue: Rat Normal Kidney.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab28374の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    特記事項
    WB
    Use at an assay dependent concentration.
    ELISA
    Use at an assay dependent concentration.
    IHC-P
    Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Play a direct role in facilitating neuronal migration.

    • 組織特異性

      Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

    • 関連疾患

      Note=Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism.

    • 配列類似性

      Belongs to the peptidase S1 family.
      Contains 1 EGF-like domain.
      Contains 1 fibronectin type-I domain.
      Contains 2 kringle domains.
      Contains 1 peptidase S1 domain.

    • ドメイン

      Both FN1 and one of the kringle domains are required for binding to fibrin.
      Both FN1 and EGF-like domains are important for binding to LRP1.
      The FN1 domain mediates binding to annexin A2.
      The second kringle domain is implicated in binding to cytokeratin-8 and to the endothelial cell surface binding site.

    • 翻訳後修飾

      The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa.
      Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin.
      N-glycosylation of Asn-152; the bound oligomannosidic glycan is involved in the interaction with the mannose receptor.
      Characterization of O-linked glycan was studied in Bowes melanoma cell line.

    • 細胞内局在

      Secreted > extracellular space.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Alteplase antibody
        • DKFZp686I03148 antibody
        • Plasminogen activator tissue antibody
        • Plasminogen activator tissue type antibody
        • PLAT antibody
        • Reteplase antibody
        • t PA antibody
        • T Plasminogen Activator antibody
        • t-PA antibody
        • T-plasminogen activator antibody
        • Tissue plasminogen activator (t PA) antibody
        • Tissue type plasminogen activator antibody
        • Tissue-type plasminogen activator chain B antibody
        • tPA antibody
        • TPA_HUMAN antibody
        • TPA1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPA Tissue Plasminogen Activator antibody [H27B6] (ab28374)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPA Tissue Plasminogen Activator antibody [H27B6] (ab28374)

        IHC image of TPA Tissue Plasminogen Activator staining in Rat Normal Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28374, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

         

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      参考文献 (4)

      ab28374 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab28374 は 4 報の論文で使用されています。

      • Cho MK  et al. Zinc-triggered induction of tissue plasminogen activator and plasminogen in endothelial cells and pericytes. Exp Neurobiol 22:315-21 (2013). WB . PubMed: 24465147
      • Hwang IY  et al. Zinc-triggered induction of tissue plasminogen activator by brain-derived neurotrophic factor and metalloproteinases. J Neurochem 118:855-63 (2011). WB . PubMed: 21615740
      • Cacquevel M  et al. Ageing and amyloid-beta peptide deposition contribute to an impaired brain tissue plasminogen activator activity by different mechanisms. Neurobiol Dis 27:164-73 (2007). PubMed: 17566751
      • Declerck PJ  et al. Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice. J Biol Chem 270:8397-400 (1995). PubMed: 7536737

      レビューと Q&A

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      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      Inquiry: We got multiple bands using this antibody and could not figure out which band is the one we want. The cell type we used is astroctyes from primary culture. 1. 35ug amount of protein was loaded. 2. Running 4-12%Gel (Invitrogen) 1and half hour at 130v and Transfer 3hrs at 80v. 3. Blocking 1 hour in 5%milk 4. 1:1000 Dilution of primary antibody, incubated overnight at 4C 5. 1:1000 Dilution of secondary antibody, incubated 1hr at RT 6. Using ECL to detect. 7. Washing 10mins×3 times after Primary and secondary antibody.

      Read More
      Answer

      I have just noticed that you were kind enough to include a western blot image on your initial email. I'm sorry to have missed that in my earlier email.

      After looking at your blot I think it might be helpful to reduce the antibody concentration of both the primary and secondary antibody. This may reduce many of these background bands. I recommend trying a 1:2000 dilution of the primary and a 1:10 000 dilution of the secondary to begin.

      I look forward to hearing if this advice proves useful.

      Read More

      Question

      Inquiry: We got multiple bands using this antibody and could not figure out which band is the one we want.

      The cell type we used is astroctyes from primary culture.
      1. 35ug amount of protein was loaded.
      2. Running 4-12%Gel (Invitrogen) 1and half hour at 130v and Transfer 3hrs at 80v.
      3. Blocking 1 hour in 5%milk
      4. 1:1000 Dilution of primary antibody, incubated overnight at 4C
      5. 1:1000 Dilution of secondary antibody, incubated 1hr at RT
      6. Using ECL to detect.
      7. Washing 10mins×3 times after Primary and secondary antibody.

      Read More
      Answer

      Thank you for your enquiry and for providing the protocol details.

      I think it would be helpful if you could provide an image of your western blot. When you have a chance, please send it along as an attachement.

      Thanks!

      Read More

      Question

      Can you please tell me if any of your Tissue Plasminogen Activator antibodies recognize only folded, active tPA or also unfolded, inactive tPA?

      Read More
      Answer

      The source of the antibody informed me " tPA does not have an active and a latent form. tPA is active in both single chain form and also once cleaved or the two chain form. If there is a latent or inactive form it is a new one on me. Your customer may be talking about a proform of tPA but, I am not familiar with this either. Our antibody for tPA has not been tested to this depth for reactivity regardless and therefore I cannot say if there is a any reactivity with the AB to folded or unfolded tPA." I hope this information will help,

      Read More

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