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AB28374

Anti-Tissue Plasminogen Activator 抗体 [H27B6]

Anti-Tissue Plasminogen Activator antibody [H27B6]

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(4 Publications)

Mouse Monoclonal Tissue Plasminogen Activator antibody. Suitable for IHC-P, ELISA, WB and reacts with Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Plat.

別名を表示する

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA, Plat

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [H27B6] (AB28374)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [H27B6] (AB28374)

IHC image of TPA Tissue Plasminogen Activator staining in Rat Normal Kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab28374, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

H27B6

アイソタイプ

IgG

キャリアフリー

No

交差種

Rat, Mouse

アプリケーション

ELISA, IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Mouse Plat.

P11214

エピトープ

protease domain (see Declerck et al)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Protein A Sepharose. Purity is = 98% assessed by visual inspection of a Coomassie® bluestained SDS-PAGE gel.
バッファー組成
pH: 7.4 Constituents: 0.87% Sodium chloride, 0.82% Sodium phosphate
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Plasminogen Activator (tPA) also known as a plasminogen activator acts mechanically to convert plasminogen to plasmin a serine protease. This reaction occurs at the surface of a fibrin clot leading to clot degradation a process known as fibrinolysis. tPA has a molecular mass of approximately 70 kDa. It is mainly expressed in vascular endothelial cells and is released into the bloodstream in response to stimuli such as circulatory stasis or endothelial damage.
Biological function summary

TPA plays a critical role in thrombolysis by breaking down blood clots into their soluble components. It regulates plasminogen function by cleaving this zymogen to yield the active protease plasmin. This function makes tPA integral in maintaining hemostasis and it does not form a part of a larger protein complex. The activity and the regulation of tPA are important for preventing pathologic clotting which can lead to cardiovascular complications.

Pathways

TPA is central to the fibrinolytic pathway. This pathway facilitates the conversion of plasminogen to plasmin enabling clot resolution. In addition tPA interacts with other proteins such as urokinase plasminogen activator (uPA) and inhibitors like plasminogen activator inhibitor-1 (PAI-1). The balance between tPA and its inhibitors is important for the regulation of fibrinolytic activity impacting hemostatic and thrombotic events.

TPA connects closely with conditions like stroke and myocardial infarction due to its thrombolytic properties. In ischemic stroke excessive or insufficient tPA activity can disrupt normal blood flow leading to tissue damage. Additionally in myocardial infarction tPA's role in breaking down clots proves important for restoring coronary blood flow. It is also linked with proteins like fibrinogen as they serve as substrates in the clot degradation process and with PAI-1 which modulates its activity and influences disease progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy (By similarity).
See full target information Plat
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental neurobiology 22:315-21 PubMed24465147

2013

Zinc-triggered induction of tissue plasminogen activator and plasminogen in endothelial cells and pericytes.

Applications

WB

Species

Unspecified reactive species

Mun-Kyung Cho,Eun-Sun Sun,Yang-Hee Kim

Journal of neurochemistry 118:855-63 PubMed21615740

2011

Zinc-triggered induction of tissue plasminogen activator by brain-derived neurotrophic factor and metalloproteinases.

Applications

WB

Species

Unspecified reactive species

Ih-Yeon Hwang,Eun-Sun Sun,Ji Hak An,Hana Im,Sun-Ho Lee,Joo-Yong Lee,Pyung-Lim Han,Jae-Young Koh,Yang-Hee Kim

Neurobiology of disease 27:164-73 PubMed17566751

2007

Ageing and amyloid-beta peptide deposition contribute to an impaired brain tissue plasminogen activator activity by different mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Cacquevel,Séverine Launay,Hervé Castel,Karim Benchenane,Simon Chéenne,Luc Buée,Lieve Moons,André Delacourte,Peter Carmeliet,Denis Vivien

The Journal of biological chemistry 270:8397-400 PubMed7536737

1995

Generation of monoclonal antibodies against autologous proteins in gene-inactivated mice.

Applications

Unspecified application

Species

Unspecified reactive species

P J Declerck,P Carmeliet,M Verstreken,F De Cock,D Collen
View all publications
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代替製品

Primary Antibodies

AB157469

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] (AB157469)

  • 3
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Primary Antibodies

AB227069

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody (AB227069)

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Primary Antibodies

AB62763

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Primary Antibodies

AB51518

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Primary Antibodies

AB48649

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Primary Antibodies

AB240146

Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Plasminogen Activator antibody [EPR7232(2)] - BSA and Azide free (AB240146)

  • 0
  • 0

組合せ製品

Primary Antibodies

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BOND RX™ Validated

Western blot - Anti-PAI1 antibody (AB66705)

  • 4
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Primary Antibodies

AB92572

Anti-Fibrinogen alpha chain antibody [EPR2919]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibrinogen alpha chain antibody [EPR2919] (AB92572)

  • 1
  • 1
Primary Antibodies

AB169754

Anti-uPA antibody [EP6274(2)]

RabMAb|Recombinant

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  • 1
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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