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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Tissue Factor 抗体 [EPR20369] (ab211016)

Submit a review Submit a question References (1)
Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
  • Anti-Tissue Factor antibody [EPR20369] (ab211016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20369] to Tissue Factor
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Tissue Factor antibody [EPR20369]
    Tissue Factor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR20369] to Tissue Factor

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human Tissue Factor His-tagged recombinant protein (aa34-251); MDA-MB-231 and U-87 MG whole cell lysates; Human fetal kidney and lung cancer lysates. IHC-P: Human placenta. kidney and pancreas cancer tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab211016の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

  • 配列類似性

    Belongs to the tissue factor family.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD142 antibody
      • CD142 antigen antibody
      • Coagulation factor III (thromboplastin tissue factor) antibody
      • Coagulation factor III antibody
      • F3 antibody
      • FLJ17960 antibody
      • TF antibody
      • TF_HUMAN antibody
      • TFA antibody
      • Thromboplastin antibody
      • Tissue factor antibody
      see all

    画像

    • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      All lanes : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution (Anti-Tissue Factor antibody NPD)

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : Tissue Factor knockout HAP1 whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Predicted band size: 33 kDa
      Observed band size: 39 kDa why is the actual band size different from the predicted?



      Lanes 1 - 2: Merged signal (red and green). Green - ab211016 observed at 49 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab211016 was shown to recognize Tissue Factor in wild-type HAP1 cells as signal was lost at the expected MW in Tissue Factor knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and F3 (Tissue Factor) knockout samples were subjected to SDS-PAGE. Ab211016 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Cytoplasmic staining on human kidney glomerulus is observed [PMID: 2237820].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Cytoplasmic staining on human placenta is observed [PMID: 2237820].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution + Human Tissue Factor His-tagged recombinant protein (aa34-251) at 0.01 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 33 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      All lanes : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/5000 dilution

      Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 33 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 15 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      MCF7 is a negative control (PMID: 24137414).

    • Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Western blot - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Lanes 1-2 : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/1000 dilution
      Lane 3 : Anti-Tissue Factor antibody [EPR20369] (ab211016) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human lung cancer lysate
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 33 kDa
      Observed band size: 42 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The observed molecular weight is consistent with the literature (PMID: 7992837).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tissue Factor antibody [EPR20369] (ab211016)

      Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue labeling Tissue Factor with ab211016 at 1/50 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Membranous and cytoplasmic staining on tumor cells of human pancreas cancer is observed [PMID: 7648165].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) Ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Tissue Factor antibody [EPR20369] (ab211016)
      Anti-Tissue Factor antibody [EPR20369] (ab211016)

    参考文献 (1)

    ab211016 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab211016 は 1 報の論文で使用されています。

    • Das K  et al. Protease-activated receptor 2 promotes actomyosin dependent transforming microvesicles generation from human breast cancer. Mol Carcinog 57:1707-1722 (2018). PubMed: 30129687

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