Anti-Tissue Factor 抗体 [EPR18160-175] (ab189483)
![Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)](https://www.abcam.co.jp/ps/products/189/ab189483/Images/ab189483-291285-anti-tissue-factor-antibody-epr18160-175-western-blot.jpg "Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18160-175] to Tissue Factor
- Suitable for: IP, WB, ICC/IF
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-Tissue Factor antibody [EPR18160-175]
Tissue Factor 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18160-175] to Tissue Factor -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Mouse skin and brain tissue lysate; NIH/3T3 whole cell lysate. IP: Mouse brain tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18160-175 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab189483の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/30.
|
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| WB |
1/1000. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa).
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| 特記事項 |
|---|
|
IP
1/30. |
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WB
1/1000. Detects a band of approximately 33, 47 kDa (predicted molecular weight: 33 kDa). |
ターゲット情報
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機能
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
配列類似性
Belongs to the tissue factor family. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 14066 Mouse
- SwissProt: P20352 Mouse
- Unigene: 273188 Mouse
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別名
- CD142 antibody
- CD142 antigen antibody
- Coagulation factor III (thromboplastin tissue factor) antibody
see all
画像
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Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)All lanes : Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : Mouse brain tissue lysate
Lanes 3-4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33, 47 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST.
Exposure: Lanes 1 and 3: 3 minutes; Lanes 2 and 4: 10 seconds.
The observed molecular mass is consistent with the literature (PMID: 7992837).
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![Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)](https://www.abcam.co.jp/ps/products/189/ab189483/Images/ab189483-291292-anti-tissue-factor-antibody-epr18160-175-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain lysate 10 µg (Input).
Lane 2: ab189483 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Anti-Tissue Factor antibody [EPR18160-175] (ab189483)](https://www.abcam.co.jp/ps/products/189/ab189483/Images/ab189483-3-benefits-of-recombinant-antibodies.png)
Anti-Tissue Factor antibody [EPR18160-175] (ab189483)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab189483 は 3 報の論文で使用されています。
- Zhang Y et al. Inflammasome activation promotes venous thrombosis through pyroptosis. Blood Adv 5:2619-2623 (2021). PubMed: 34152402
- Zheng B et al. Lidocaine Alleviates Sepsis-Induced Acute Lung Injury in Mice by Suppressing Tissue Factor and Matrix Metalloproteinase-2/9. Oxid Med Cell Longev 2021:3827501 (2021). PubMed: 34804364
- Rosell A et al. Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting. Res Pract Thromb Haemost 4:1013-1023 (2020). PubMed: 32864552