AB189483

Anti-Tissue Factor 抗体 [EPR18160-175]

Anti-Tissue Factor antibody [EPR18160-175]

RabMAb
Recombinant
詳細を見る

Be the first to review this product! Submit a review

(6 Publications)

Rabbit Recombinant Monoclonal Tissue Factor antibody. Suitable for ICC/IF, IP, WB and reacts with Mouse samples. Cited in 6 publications.

別名を表示する

CD142, Cf-3, Cf3, F3, Tissue factor, TF, Coagulation factor III

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Tissue Factor with ab189483 at 1/50 (11.52 µg/ml) dilution, followed by ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green).

Confocal image showing cytoplasmic staining in NIH/3T3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/ml) dilution.

Supplier Data

Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

Tissue Factor was immunoprecipitated from 0.35 mg of mouse brain lysate with ab189483 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab189483 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Mouse brain lysate 10 μg (Input).

Lane 2 : ab189483 IP in mouse brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab1189483 in mouse brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (ab189483)

Predicted band size: 33 kDa

Observed band size: 47 kDa

true

Supplier Data

Western blot - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

Blocking/Dilution : 5% NFDM/TBST.

Exposure : Lanes 1 and 3 : 3 minutes; Lanes 2 and 4 : 10 seconds.

The observed molecular mass is consistent with the literature (PMID : 7992837).

All lanes:

Western blot - Anti-Tissue Factor antibody [EPR18160-175] (ab189483) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lanes 3 - 4:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa,47 kDa

true

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18160-175

アイソタイプ

IgG

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF, IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Tissue Factor also known as coagulation factor III or CD142 is an important player in the blood coagulation process. This protein with a molecular mass of approximately 47 kDa is a transmembrane glycoprotein. It is expressed widely in various tissues including the lungs heart and brain and plays an important role in initiating the coagulation cascade when exposed to blood. In endothelial cells tissue factor is generally not present unless these cells are in a state of disturbance supporting its role as a primary defense mechanism against vascular injury.

Biological function summary

Tissue factor acts as a cofactor for factor VIIa forming a complex that rapidly catalyzes the activation of factor X to factor Xa. This catalytic action ultimately leads to fibrin formation which is essential for clotting. Tissue Factor (TF) is part of the extrinsic pathway of the coagulation cascade and is fundamental in the generation of a hemostatic response to tissue injury. By regulating thrombin generation through its interaction with other coagulation factors tissue factor plays an important role in maintaining hemostatic balance.

Pathways

Tissue factor is integral in the extrinsic pathway of coagulation which works closely with the intrinsic pathway to ensure effective hemostasis. It complexes with factor VIIa and triggers downstream effects involving proteins like factor X and thrombin. Tissue factor forms a bridge between cellular injury responses and the blood coagulation system linking these two significant physiological processes. The interaction with these proteins highlights its central role in hemostatic pathways and highlights its significance in the coagulation network.

Tissue factor is associated with conditions like thrombosis and cardiovascular diseases where its expression can become dysregulated. Abnormal activation of tissue factor contributes directly to the pathological clot formation seen in thrombosis. It is also implicated in cancer-related coagulopathy where it can facilitate tumor-associated thrombosis by interacting with cancer cell surfaces. Additionally in such diseases the interplay between tissue factor and thrombin becomes important as it affects disease progression and the severity of clot-related complications.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF : VIIa] complex activates factors IX or X by specific limited proteolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

See full target information F3

style

left-column

文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 27:138 PubMed40713647

2025

Short-term consumption of the modified standard American diet perturbed the metabolic balance and altered DNA damage in MMTV-PyMT transgenic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Arlet Hernandez,Alekhya Puppala,Jenna Hedlich-Dwyer,Nayonika Mukherjee,Guihua Zhai,Valeria L Dal Zotto,Bohan Ning,Hua Guo,Ritu Aneja,Natalie R Gassman

Research and practice in thrombosis and haemostasis 8:102325 PubMed38404939

2024

Evaluation of the ability of commercial enzyme-linked immunosorbent assays to measure mouse tissue factor.

Applications

Unspecified application

Species

Unspecified reactive species

Ana T A Sachetto,Nigel Mackman

iScience 25:104547 PubMed35754738

2022

Coagulation factors promote brown adipose tissue dysfunction and abnormal systemic metabolism in obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Hayashi,Ippei Shimizu,Yohko Yoshida,Ryutaro Ikegami,Masayoshi Suda,Goro Katsuumi,Shinya Fujiki,Kazuyuki Ozaki,Manabu Abe,Kenji Sakimura,Shujiro Okuda,Toshiya Hayano,Kazuhiro Nakamura,Kenneth Walsh,Naja Zenius Jespersen,Søren Nielsen,Camilla Scheele,Tohru Minamino

Oxidative medicine and cellular longevity 2021:3827501 PubMed34804364

2021

Lidocaine Alleviates Sepsis-Induced Acute Lung Injury in Mice by Suppressing Tissue Factor and Matrix Metalloproteinase-2/9.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Zheng,Hongbo Yang,Jianan Zhang,Xueli Wang,Hao Sun,Fan Hu,Qian Li,Liping Jiang,Yue Su,Qilin Peng,Yulin Tang,Wen-Tao Liu,Xueming He,Yixin Fan,Xia Zhu

Blood advances 5:2619-2623 PubMed34152402

2021

Inflammasome activation promotes venous thrombosis through pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Jian Cui,Guoying Zhang,Congqing Wu,Ahmed Abdel-Latif,Susan S Smyth,Toshihiko Shiroishi,Nigel Mackman,Yinan Wei,Min Tao,Zhenyu Li

Research and practice in thrombosis and haemostasi 4:1013-1023 PubMed32864552

2020

Evaluation of different commercial antibodies for their ability to detect human and mouse tissue factor by western blotting.

Applications

Unspecified application

Species

Unspecified reactive species

Axel Rosell,Bernhard Moser,Yohei Hisada,Rukesh Chinthapatla,Grace Lian,Yi Yang,Matthew J Flick,Nigel Mackman

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tissue Factor 抗体 [EPR18160-175]

Anti-Tissue Factor antibody [EPR18160-175]

Immunocytochemistry/ Immunofluorescence - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

Immunoprecipitation - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

Western blot - Anti-Tissue Factor antibody [EPR18160-175] (AB189483)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (6)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Abcam product promise