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Anti-TIS11D 抗体 (ab70775)

Submit a review Q&A (3)References (4)
Western blot - Anti-TIS11D antibody (ab70775)

    Key features and details

    • Rabbit polyclonal to TIS11D
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-TIS11D antibody

    • 製品の詳細

      Rabbit polyclonal to TIS11D

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide corresponding to Human TIS11D (internal sequence).
      Database link: P47974

    • ポジティブ･コントロール

      • A549 cell extracts.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab70775の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).
    特記事項
    WB
    1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 52 kDa).

    ターゲット情報

    • 機能

      mRNA-binding protein that plays a key role in self-renewal of erythroid cells in response to glucocorticoids. Specifically binds to the AU-rich element (ARE) in the 3'-UTR of target mRNAs, promoting their deadenylation and degradation (PubMed:14981510). Specifically expressed in burst-forming unit-erythroid (BFU-E) progenitors in response to glucocorticoids and acts as a negative regulator of erythroid cell differentiation: promotes self-renewal of erythroid cells by binding mRNAs that are induced or highly expressed during terminal erythroid differentiation and promotes their degradation, preventing erythroid cell differentiation. Down-regulated during erythroid differentiation from the BFU-E stage, stabilizing mRNAs required for terminal differentiation.

    • 関連疾患

      Defects in ZFP36L2 may be a cause of leukemias. Frameshfits mutations disrupting ZFP36L2 have been found in a patient with acute myeloid leukemia (PubMed:21109922).

    • 配列類似性

      Contains 2 C3H1-type zinc fingers.

    • 細胞内局在

      Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BRF2 antibody
        • Butyrate response factor 2 antibody
        • C3H1 type-like 2 antibody
        • EGF response factor 2 antibody
        • EGF-response factor 2 antibody
        • ERF 2 antibody
        • ERF-2 antibody
        • ERF2 antibody
        • Protein TIS11D antibody
        • RNF162C antibody
        • TIS11D antibody
        • TISD_HUMAN antibody
        • ZFP36 like 2 antibody
        • ZFP36 ring finger protein like 2 antibody
        • ZFP36-like 2 antibody
        • Zfp36l2 antibody
        • Zinc finger protein 36 antibody
        • Zinc finger protein 36, C3H type like 1 antibody
        • zinc finger protein 36, C3H type like 2 antibody
        • Zinc finger protein 36, C3H1 type like 2 antibody
        • Zinc finger protein, C3H type, 36 like 2 antibody
        see all

      画像

      • Western blot - Anti-TIS11D antibody (ab70775)
        Western blot - Anti-TIS11D antibody (ab70775)
        All lanes : Anti-TIS11D antibody (ab70775) at 1/500 dilution

        Lane 1 : A549 cell extract
        Lane 2 : A549 cell extract with immunising peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 52 kDa
        Observed band size: 42 kDa why is the actual band size different from the predicted?

      参考文献 (4)

      ab70775 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab70775 は 4 報の論文で使用されています。

      • Trivedi G  et al. Muscarinic acetylcholine receptor regulates self-renewal of early erythroid progenitors. Sci Transl Med 11:N/A (2019). PubMed: 31554738
      • Salerno F  et al. Translational repression of pre-formed cytokine-encoding mRNA prevents chronic activation of memory T cells. Nat Immunol N/A:N/A (2018). PubMed: 29988089
      • Dumdie JN  et al. Chromatin Modification and Global Transcriptional Silencing in the Oocyte Mediated by the mRNA Decay Activator ZFP36L2. Dev Cell 44:392-402.e7 (2018). PubMed: 29408237
      • Zhang L  et al. ZFP36L2 is required for self-renewal of early burst-forming unit erythroid progenitors. Nature 499:92-6 (2013). WB, IP ; Mouse . PubMed: 23748442

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      I have asked for "sample size" antibody, if you have them available. The "100 ug" is a full-size vial which would suffice for at least a dozen of WB applications. We just would like to see how the Ab worked and if they would distinguish the isoforms (ZFP36, L1 and L2) before buying the full size.

      Read More
      Answer

      Thank you for your reply.

      I have heard back from the lab regarding ab83579:


      Crossreactivity with TISIIB and TISIID isn't something we have tested in house but an immunogen search shows that both TISIIB and TISIID only have 4 residues of the immunogen in common so we would say it is very unlikely that ab83579 would detect these 2 proteins.
      We do not offer smaller samples sizes than our selling size (due to operational resons), but have a guarantee in place that covers the antibody for 6 months after the purchase for the use in the tested species and applications as listed on the datasheet (https://www.abcam.com/Abpromise).
      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      cross reactivity with TIS11A, TIS11B and TIS11D determined?

      Read More
      Answer

      Thank you for contacting us.

      I have received the following information regading 2 of the antibodies. For ab83579, I am still waiting for more information.

      ab70775:
      The antibody was raised against human TIS11D. This antibody has been validated for Western blot using A549 cell extracts. This antibody has not been tested against TIS11A or TIS11B. Alignment of the antigen sequence with TIS11A (http://www.uniprot.org/uniprot/P2665) shows a 60% homology.
      Alignment of the antigen sequence with TIS11B (http://www.uniprot.org/uniprot/Q07352) shows a 66.7% homology.

      ab53747:
      We have not obtained data from endogenous protein and have not tested cross reactivity with TIS11A and TIS11D.
      However, we checked cross reactivity using “BLAST search” between immunogen sequence of ab53747 and TIS11A, TIS11D. And the results are the homology is for TIS11D: 76%, and for TIS11A: 68%. Crossreactivity might be possible for TIS11D.

      I will be in touch with more information regarding ab83579.

      I hope this information is already of help to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      NInquiry: You have the wrong size for this protein --you note this but do not provide an explanation. Is the immunogen sequence in the region that is distinct from Tis11b --our antibody against Tis11b recognizes both --we need to confirm this

      Read More
      Answer

      Thank you for contacting us.





      The immunogen sequence used to create this antibody is from aa231-270 ofhuman TIS11D. This sequence contains a 15aa span which has a 70% homology with TIS11B. At this level, we do not consider ab70775 to be cross reactive with TIS11B.

      Western blot is smaller than the band predicted by sequence. However, migration is affected by other factors and so the actual band size observed may differ from that predicted. Common factors include...

      post-translational modification- e.g. phosphorylation, glycosylation etc which increases the size of the protein
      post-translation cleavage- e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases
      splice variants- alternative splicing may create different sized proteins from the same gene
      relative charge- the composition of amino acids (charged vs non-charged)



      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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