Anti-TIP49A 抗体 [2943C1a] (ab51500)
![Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.co.jp/ps/products/51/ab51500/Images/ab51500-63450-anti-tip49a-antibody-2943c1a-western-blot.jpg "Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)")
Key features and details
- Mouse monoclonal [2943C1a] to TIP49A
- Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-TIP49A antibody [2943C1a]
TIP49A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2943C1a] to TIP49A -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, internal sequence. (Human)
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ポジティブ･コントロール
- HT-1080 whole cell lysate, HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.1312% Sodium phosphate, 1.05% Potassium chloride, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
ab51500 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
ポリ/モノ
モノクローナル -
クローン名
2943C1a -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51500の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.5 µg/ml.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use at an assay dependent concentration.
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| WB | (3) |
1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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Flow Cyt
Use a concentration of 0.5 µg/ml. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex.
May be able to bind plasminogen at cell surface and enhance plasminogen activation.
Essential for cell proliferation. -
組織特異性
Ubiquitously expressed with high expression in heart, skeletal muscle and testis. -
配列類似性
Belongs to the ruvB family. -
ドメイン
Binding to MYC is dependent on a Myc domain essential for oncogenic activity. -
細胞内局在
Nucleus matrix. Nucleus > nucleoplasm. Cytoplasm. Membrane. Cytoplasm > cytoskeleton > centrosome. Mainly localized in the nucleus, associated with nuclear matrix or in the nuclear cytosol, although it is also present in the cytoplasm and associated with the cell membranes. In prophase and prometaphase it is located at the centrosome and the branching microtubule spindles. After mitotic nuclear membrane disintigration it accumulates at the centrosome and sites of tubulin polymerization. As cells pass through metaphase and into telophase it is located close to the centrosome at the early phase of tubulin polymerization. In anaphase it accumulates at the zone of tubule interdigitation. In telophase it is found at polar tubule overlap, and it reappears at the site of chromosomal decondensation in the daughter cells. - Information by UniProt
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参照データベース
- Entrez Gene: 8607 Human
- Entrez Gene: 56505 Mouse
- Omim: 603449 Human
- SwissProt: Q9Y265 Human
- SwissProt: P60122 Mouse
- Unigene: 272822 Human
- Unigene: 42195 Mouse
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別名
- 49 kDa TATA box binding protein interacting protein antibody
- 49 kDa TATA box-binding protein-interacting protein antibody
- 49 kDa TBP interacting protein antibody
see all
画像
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Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg
Secondary
Anti Mouse IgG at 1/2500 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa -
![Flow Cytometry - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.co.jp/ps/products/51/ab51500/Images/ab51500-63449-anti-tip49a-antibody-2943c1a-flow-cytometry.jpg)
Flow Cytometry - Anti-TIP49A antibody [2943C1a] (ab51500)Flow cytometry using ab51500 at 0.5ug using HeLa cells. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg). White peak shows an isotype control, while the red peak shows ab51500. -
![Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.co.jp/ps/products/51/ab51500/Images/ab51500-63448-anti-tip49a-antibody-2943c1a-western-blot.jpg)
Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)This image is courtesy of an anonymous AbreviewAll lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/ml (in 3% BSA + PBS-Tween 0.1% for 12 hours at 4°C)
Lanes 1-2 : HeLa cell lysate + control siRNA sequence
Lane 3 : HeLa cell lysate suppressed by siRNA (1) sequence to pontin
Lane 4 : HeLa cell lysate suppressed by siRNA (2) sequence to pontin
Lane 5 : Cell lysate of 293T cells
Lane 6 : Cell lysate of HeLa cells
Lane 7 : Cell lysate of U251 cells
Lane 8 : Cell lysate of Jurkat cells
Lane 9 : Cell lysate of U2OS cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 3% BSA for 1 hour at 21°C -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.co.jp/ps/products/51/ab51500/Images/ab51500-63447-anti-tip49a-antibody-2943c1a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [2943C1a] (ab51500)IHC image of ab51500 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51500, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.co.jp/ps/products/51/ab51500/Images/ab51500-63446-anti-tip49a-antibody-2943c1a-western-blot.jpg)
Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)Image courtesy of an anonymous Abreview.All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC-12 (Rat adrenal gland pheochromocytoma) cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Excellent antibody. Detects a single species of the predicted mobility in a range of common rat cell lines.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab51500 は 13 報の論文で使用されています。
- Papin C et al. MBD4 loss results in global reactivation of promoters and retroelements with low methylated CpG density. J Exp Clin Cancer Res 42:301 (2023). PubMed: 37957685
- Tang D et al. Pontin Functions as A Transcriptional Co-activator for Retinoic Acid-induced HOX Gene Expression. J Mol Biol 433:166928 (2021). PubMed: 33713676
- Li N et al. Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair. Nucleic Acids Res 48:3014-3028 (2020). PubMed: 31980815
- MacNeil DE et al. N-terminal residues of human dyskerin are required for interactions with telomerase RNA that prevent RNA degradation. Nucleic Acids Res 47:5368-5380 (2019). PubMed: 30931479
- Runge JS et al. Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide. G3 (Bethesda) 8:1095-1102 (2018). PubMed: 29432129