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リコンビナントRabMAb

Anti-TIP30 抗体 [EPR13179] (ab177961)

Submit a review Submit a question References (4)
Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)
  • Anti-TIP30 antibody [EPR13179] (ab177961)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13179] to TIP30
  • Suitable for: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TIP30 antibody [EPR13179]
    TIP30 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13179] to TIP30

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), IP, IHC-P, WB, ICC/IFmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human TIP30 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9BUP3

  • ポジティブ･コントロール

    • HeLa, A431, and fetal liver lysates; Human pancreas and placenta tissues; A431 cells; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab177961の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC/IF
1/50 - 1/100.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC/IF
1/50 - 1/100.

ターゲット情報

  • 機能

    Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import. Isoform 1 is a metastasis suppressor with proapoptotic as well as antiangiogenic properties. Isoform 2 has an antiapoptotic effect.

  • 組織特異性

    Ubiquitous. Highest level in liver. High levels in lung, skeletal muscle, pancreas and placenta. Moderate levels in heart and kidney. Low levels in brain. Not expressed or low levels in variant small cell lung carcinomas, 33% of hepatocellular carcinomas and neuroblastomas.

  • ドメイン

    Unique C-terminus confers high proteasome-dependent instability to isoform 2.

  • 細胞内局在

    Cytoplasm. Nucleus envelope.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 30 kDa HIV 1 TAT interacting protein antibody
      • 30 kDa HIV-1 TAT-interacting protein antibody
      • 30 kDa HIV1 TAT interacting protein antibody
      • CC3 antibody
      • FLJ26963 antibody
      • HIV 1 Tat interacting protein 2 30kDa antibody
      • HIV 1 Tat interacting protein 2 antibody
      • HIV-1 TAT-interactive protein 2 antibody
      • HTAI2_HUMAN antibody
      • HTATIP 2 antibody
      • HTATIP2 antibody
      • Oxidoreductase HTATIP2 antibody
      • SDR44U1 antibody
      • Short chain dehydrogenase/reductase family 44U member 1 antibody
      • Tat interacting protein (30kDa) antibody
      see all

    画像

    • Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
      Western blot - Anti-TIP30 antibody [EPR13179] (ab177961)
      All lanes : Anti-TIP30 antibody [EPR13179] (ab177961) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : Fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 27 kDa

    • Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Flow Cytometry (Intracellular) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Intracellular flow cytometric analysis of permeabilized HeLa cells labeling TIP30 using ab177961 at 1/10 dilution (red) or a rabbit IgG (negative) (green). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunocytochemistry/ Immunofluorescence - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunofluorescence analysis of A431 cells labeling TIP30 using ab177961 at 1/50 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunohistochemical analysis of Human placenta tissue labeling TIP30 using ab177961 at 1/50 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP30 antibody [EPR13179] (ab177961)

      Immunohistochemical analysis of Human pancreas tissue labeling TIP30 using ab177961 at 1/50 dilution.

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)
      Immunoprecipitation - Anti-TIP30 antibody [EPR13179] (ab177961)

      Western blot analysis on immunoprecipitation pellet from (1) HeLa cell lysate or (2) 1XPBS (negative control) using ab177961 at 1/10 dilution to immunoprecipitate TIP30, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Anti-TIP30 antibody [EPR13179] (ab177961)
      Anti-TIP30 antibody [EPR13179] (ab177961)

    参考文献 (4)

    ab177961 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab177961 は 4 報の論文で使用されています。

    • Lin X  et al. Long Non-Coding RNA NONHSAT076754 Promotes Invasion and Metastasis in Epithelial Ovarian Cancer. J Cancer 10:1930-1940 (2019). PubMed: 31205552
    • Grund A  et al. TIP30 counteracts cardiac hypertrophy and failure by inhibiting translational elongation. EMBO Mol Med 11:e10018 (2019). PubMed: 31468715
    • Yang Q  et al. Metformin Enhances the Effect of Regorafenib and Inhibits Recurrence and Metastasis of Hepatic Carcinoma After Liver Resection via Regulating Expression of Hypoxia Inducible Factors 2a (HIF-2a) and 30 kDa HIV Tat-Interacting Protein (TIP30). Med Sci Monit 24:2225-2234 (2018). PubMed: 29654226
    • You A  et al. Metformin sensitizes sorafenib to inhibit postoperative recurrence and metastasis of hepatocellular carcinoma in orthotopic mouse models. J Hematol Oncol 9:20 (2016). WB ; Human . PubMed: 26957312

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