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AB239012

Anti-Tin2 抗体 [EPR15319] - BSA and Azide free

Anti-Tin2 antibody [EPR15319] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Tin2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

別名を表示する

TIN2, TINF2, TERF1-interacting nuclear factor 2, TRF1-interacting nuclear protein 2

1 Images
Western blot - Anti-Tin2 antibody [EPR15319] - BSA and Azide free (AB239012)
  • WB

Supplier Data

Western blot - Anti-Tin2 antibody [EPR15319] - BSA and Azide free (AB239012)

5% NFDM/TBST : Blocking and diluting buffer.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197894).

All lanes:

Western blot - Anti-Tin2 antibody [EPR15319] (<a href='/products/primary-antibodies/tin2-antibody-epr15319-ab197894'>ab197894</a>) at 1/10000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

WI38 (Human fetal lung fibroblast cells) whole cell lysate at 20 µg

Lane 3:

HUVEC (Human umbilical vein endothelial cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa

Observed band size: 39 kDa

false

Exposure time: 1min

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR15319

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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製品の詳細

ab239012 is the carrier-free version of ab197894.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
Do Not Freeze
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIN2 also known as TERF1-interacting nuclear factor 2 is a pivotal protein involved in telomere maintenance. It has a molecular mass of approximately 50 kDa. This protein is expressed in the nucleus where it plays an integral role in structuring chromosome ends. TIN2 specifically associates with telomeric regions to regulate their length and integrity. It forms connections with other proteins to facilitate its function in telomers' structural configuration.
Biological function summary

TIN2 integrates into the shelterin complex a group of six proteins that protect telomeres from being recognized as DNA damage. It acts as a stabilizer within this complex and helps maintain telomere integrity. In doing so TIN2 modulates the actions of telomerase and other telomere-binding proteins to ensure proper chromosome end function. This responsibility makes it essential for preventing excessive telomere shortening which can lead to genomic instability.

Pathways

The involvement of TIN2 is significant in telomere length regulation and DNA damage response mechanisms. TIN2 operates within these pathways to manage telomerase activity and interaction with telomere ends. It collaborates with other shelterin proteins like TRF1 and TRF2 which also participate in maintaining telomere function. TIN2's role in these pathways highlights its contribution to chromosome end protection and preventive response against DNA damage.

TIN2 dysfunction is linked to conditions like dyskeratosis congenita and aplastic anemia. These disorders are marked by defective telomere maintenance leading to progressive bone marrow failure and other cellular deficiencies. Mutations in TIN2 can perturb the function of shelterin proteins including TRF1 contributing to the compromised telomere integrity seen in these diseases. Understanding TIN2's role offers insights into therapeutic targets for managing telomere-associated disorders.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Plays a role in shelterin complex assembly. Isoform 1 may have additional role in tethering telomeres to the nuclear matrix.
See full target information TINF2
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 15:1336282 PubMed38576477

2024

PPARδ agonist protects against osteoarthritis by activating AKT/mTOR signaling pathway-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Guantong Sun,Xiaodong Li,Pengcheng Liu,Yao Wang,Cheng Yang,Shuhong Zhang,Lei Wang,Xiaoqing Wang
View all publications
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組合せ製品

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Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

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Primary Antibodies

AB195234

Anti-TPP1 antibody [EPR16537]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TPP1 antibody [EPR16537] (AB195234)

  • 3
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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