Anti-TIMP4 抗体 (ab38987)
Key features and details
- Rabbit polyclonal to TIMP4
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-TIMP4 antibody
TIMP4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIMP4 -
由来種
Rabbit -
特異性
Ab38987 recognises the carboxiterminal region of TIMP4. It does not react with other TIMPs. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Synthetic peptide based on the carboxyterminal region of human TIMP4.
Immunogen の所有権に関して (Peptide available as ab41155.) -
ポジティブ･コントロール
- Human TIMP4, cell media from human colorectal adenosarcoma and rat heart.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: 2.9% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38987の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa).
Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 23 kDa (predicted molecular weight: 26 kDa). Recommended starting dilution of 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 26 kDa. When used against the reduced protein, ab38987 identifies bands at 23 and 29 kDa, representing the unglycosylated and glycosylated forms of TIMP4 respectively. Treatment of cells with the phorbol ester TPA stimulates production of TIMP4 in some cell types but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting. Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. -
組織特異性
Abundant in heart and present at low levels in many other tissues. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 7079 Human
- Entrez Gene: 680130 Rat
- Omim: 601915 Human
- SwissProt: Q99727 Human
- SwissProt: P81556 Rat
- Unigene: 591665 Human
- Unigene: 155651 Rat
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別名
- Metalloproteinase inhibitor 4 antibody
- TIMP 4 antibody
- TIMP metallopeptidase inhibitor 4 antibody
see all
画像
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Western blot - Anti-TIMP4 antibody (ab38987)All lanes : Anti-TIMP4 antibody (ab38987) at 1/1000 dilution
Lane 1 : human TIMP4
Lane 2 : cell media from human colorectal adenosarcoma
Lane 3 : rat heart
Predicted band size: 26 kDa
Observed band size: 25,45,66,97 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 23 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab38987 は 2 報の論文で使用されています。
- Corporan D et al. Temporal changes in myocardial collagen, matrix metalloproteinases, and their tissue inhibitors in the left ventricular myocardium in experimental chronic mitral regurgitation in rodents. Am J Physiol Heart Circ Physiol 315:H1269-H1278 (2018). PubMed: 30141979
- Horn MA et al. Age-related divergent remodeling of the cardiac extracellular matrix in heart failure: collagen accumulation in the young and loss in the aged. J Mol Cell Cardiol 53:82-90 (2012). WB ; Sheep . PubMed: 22516365
