Anti-TIMP3 抗体 (ab39184)
Key features and details
- Rabbit polyclonal to TIMP3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TIMP3 antibody
TIMP3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIMP3 -
由来種
Rabbit -
特異性
ab39184 binds to TIMP3. It recognizes the glycosylated and unglycosylated forms of TIMP3, and works against native or reduced TIMP3. Ab39184 does not cross react with the other TIMP family members (TIMP1, TIMP2, TIMP4). -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide of TIMP3 based on loop #1 of the human TIMP3.
Immunogen の所有権に関して (Peptide available as ab41152.) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39184の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 1 - 5 µg/ml.
|
| WB | (3) |
1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection.
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
WB
1/1000 - 1/5000. Detects a band of approximately 24, 30 kDa (predicted molecular weight: 24 kDa). Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. -
関連疾患
Defects in TIMP3 are the cause of Sorsby fundus dystrophy (SFD) [MIM:136900]. SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 7078 Human
- Entrez Gene: 21859 Mouse
- Omim: 188826 Human
- SwissProt: P35625 Human
- SwissProt: P39876 Mouse
- Unigene: 644633 Human
- Unigene: 714168 Human
- Unigene: 4871 Mouse
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別名
- HSMRK222 antibody
- K222 antibody
- K222TA2 antibody
see all
画像
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Western blot - Anti-TIMP3 antibody (ab39184)All lanes : Anti-TIMP3 antibody (ab39184)
Lane 1 : Human TIMP3
Lane 2 : Mouse TIMP3
Lane 3 : crude control (preparation of extracellular matrix from BHK cells producing recombinant human TIMP-3)
Predicted band size: 24 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-TIMP3 antibody (ab39184)ICC/IF image of ab39184 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39184, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (72)
ab39184 は 72 報の論文で使用されています。
- Park GY et al. Oxo-M and 4-PPBP Delivery via Multi-Domain Peptide Hydrogel Toward Tendon Regeneration. Front Bioeng Biotechnol 10:773004 (2022). PubMed: 35155388
- Tsuchiya K et al. m6A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer. Cancer Gene Ther 29:1355-1372 (2022). PubMed: 35318440
- Li L et al. Trehalose Protects Keratinocytes against Ultraviolet B Radiation by Activating Autophagy via Regulating TIMP3 and ATG9A. Oxid Med Cell Longev 2022:9366494 (2022). PubMed: 35450405
- Hood EMS et al. Isolation, culture, and cryosectioning of primary porcine retinal pigment epithelium on transwell cell culture inserts. STAR Protoc 3:101758 (2022). PubMed: 36227744
- Wu C et al. Tannic acid-loaded hydrogel coating endues polypropylene mesh with hemostatic and anti-inflammatory capacity for facilitating pelvic floor repair. Regen Biomater 9:rbac074 (2022). PubMed: 36267152