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AB109512

Anti-Timeless 抗体 [EPR5275]

Anti-Timeless antibody [EPR5275]

5

(1 Review)

|

(16 Publications)

Rabbit Recombinant Monoclonal Timeless antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 16 publications.

別名を表示する

TIM, TIM1, TIMELESS1, TIMELESS, Protein timeless homolog, hTIM

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] (AB109512)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Timeless antibody [EPR5275] (AB109512)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Timeless with purified ab109512 at 1/50 dilution (2.5 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (AB109512)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Timeless antibody [EPR5275] (AB109512)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling Timeless with purified ab109512 at 1/500 dilution (0.25 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Western blot - Anti-Timeless antibody [EPR5275] (AB109512)
  • WB

Unknown

Western blot - Anti-Timeless antibody [EPR5275] (AB109512)

All lanes:

Western blot - Anti-Timeless antibody [EPR5275] (ab109512) at 1/50000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

JAR (Human placenta choriocarcinoma epithelial cell) whole cell lysate

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 139 kDa

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関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-Timeless antibody [EPR5275] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5275

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250. </strong></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000", "WB-species-notes": "<p><strong>For unpurified use at 1/50000 - 1/200000.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Timeless also known as timeless circadian regulator is a protein with a molecular mass of about 139 kDa. It plays an important role in the stabilization of the circadian rhythm in mammals. Timeless associates with other clock proteins and participates in the feedback loop that drives circadian oscillations influencing hormonal and metabolic pathways. Researchers find Timeless expression primarily in the suprachiasmatic nucleus of the brain the central circadian pacemaker but it is also present in peripheral tissues.
Biological function summary

The protein influences the circadian rhythm by interacting with CLOCK and BMAL1 forming part of a macromolecular complex. Timeless helps sustain the proper timing and expression of various genes influenced by the circadian rhythm ensuring an orderly and synchronized cellular process across tissues. Its interaction with other clock components regulates circadian-controlled pathways governing processes like sleep-wake cycles hormone release and cell metabolism.

Pathways

Timeless participates in the regulation of the circadian rhythm and the cell cycle. It interacts with PER1 and PER2 proteins central to the inhibition of the CLOCK/BMAL1 complex which plays a pivotal role in maintaining the circadian clock's precision. Timeless also interfaces with proteins involved in DNA damage response pathways promoting effective DNA repair during cell division and aligning cell cycle regulation with circadian rhythms.

Timeless has been linked to cancer and sleep disorders. The protein's role in DNA repair and cell cycle control associates it with tumorigenesis when its function becomes dysregulated. Additionally disruptions in Timeless expression or function are connected to sleep phase disorders highlighting its involvement in maintaining proper circadian function. In cancer alterations in Timeless could lead to interactions with oncogenes or tumor suppressors potentially affecting cancer progression.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Plays an important role in the control of DNA replication, maintenance of replication fork stability, maintenance of genome stability throughout normal DNA replication, DNA repair and in the regulation of the circadian clock (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 23418588, PubMed : 26344098, PubMed : 31138685, PubMed : 32705708, PubMed : 35585232, PubMed : 9856465). Required to stabilize replication forks during DNA replication by forming a complex with TIPIN : this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress (PubMed : 17141802, PubMed : 17296725, PubMed : 23359676, PubMed : 35585232). During DNA replication, inhibits the CMG complex ATPase activity and activates DNA polymerases catalytic activities, coupling DNA unwinding and DNA synthesis (PubMed : 23359676). TIMELESS promotes TIPIN nuclear localization (PubMed : 17141802, PubMed : 17296725). Plays a role in maintaining processive DNA replication past genomic guanine-rich DNA sequences that form G-quadruplex (G4) structures, possibly together with DDX11 (PubMed : 32705708). Involved in cell survival after DNA damage or replication stress by promoting DNA repair (PubMed : 17141802, PubMed : 17296725, PubMed : 26344098, PubMed : 30356214). In response to double-strand breaks (DSBs), accumulates at DNA damage sites and promotes homologous recombination repair via its interaction with PARP1 (PubMed : 26344098, PubMed : 30356214, PubMed : 31138685). May be specifically required for the ATR-CHEK1 pathway in the replication checkpoint induced by hydroxyurea or ultraviolet light (PubMed : 15798197). Involved in the determination of period length and in the DNA damage-dependent phase advancing of the circadian clock (PubMed : 23418588, PubMed : 31138685). Negatively regulates CLOCK|NPAS2-ARTNL/BMAL1|ARTNL2/BMAL2-induced transactivation of PER1 possibly via translocation of PER1 into the nucleus (PubMed : 31138685, PubMed : 9856465). May play a role as destabilizer of the PER2-CRY2 complex (PubMed : 31138685). May also play an important role in epithelial cell morphogenesis and formation of branching tubules (By similarity).
See full target information TIMELESS
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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115114 PubMed39729394

2024

Identification of modulators of the ALT pathway through a native FISH-based optical screen.

Applications

Unspecified application

Species

Unspecified reactive species

Benura Azeroglu,Simran Khurana,Shih-Chun Wang,Gianna M Tricola,Shalu Sharma,Camille Jubelin,Ylenia Cortolezzis,Gianluca Pegoraro,Kyle M Miller,Travis H Stracker,Eros Lazzerini Denchi

Molecular carcinogenesis 63:1599-1610 PubMed38818892

2024

KNTC1 knockdown inhibits the proliferation and migration of osteosarcoma cells by MCM2.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhong,Yuanwei Dong,Shuqin Liu

Nature 628:433-441 PubMed38509368

2024

Transcription-replication conflicts underlie sensitivity to PARP inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Michalis Petropoulos,Angeliki Karamichali,Giacomo G Rossetti,Alena Freudenmann,Luca G Iacovino,Vasilis S Dionellis,Sotirios K Sotiriou,Thanos D Halazonetis

PloS one 19:e0298295 PubMed38354174

2024

SP3-induced Timeless transcription contributes to cell growth of lung adenocarcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Tian,Dajun Du,Li Yang,Nan Zhou,Ling Tao

European journal of medical research 28:563 PubMed38053143

2023

An integrative evaluation of circadian gene TIMELESS as a pan-cancer immunological and predictive biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Yaocheng Yang,Xianzhe Tang,Zhengjun Lin,Tao Zheng,Sheng Zhang,Tang Liu,Xiaolun Yang

BMC medicine 21:417 PubMed37924048

2023

Six-hour time-restricted feeding inhibits lung cancer progression and reshapes circadian metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Shi,Gaofeng Fang,Qianyao Chen,Jianling Li,Xiongzhong Ruan,Xuemei Lian

Journal of translational medicine 21:400 PubMed37340461

2023

TIMELESS upregulates PD-L1 expression and exerts an immunosuppressive role in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinrui Dong,Huijuan Dai,Yanping Lin,Xiaonan Sheng,Ye Li,Yaohui Wang,Xueli Zhang,Shuheng Jiang,Wenjin Yin,Jinsong Lu

Nature communications 14:1791 PubMed36997515

2023

Excessive reactive oxygen species induce transcription-dependent replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Andrs,Henriette Stoy,Barbora Boleslavska,Nagaraja Chappidi,Radhakrishnan Kanagaraj,Zuzana Nascakova,Shruti Menon,Satyajeet Rao,Anna Oravetzova,Jana Dobrovolna,Kalpana Surendranath,Massimo Lopes,Pavel Janscak

Stem cells international 2023:2826815 PubMed37964983

2023

A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin Liu

Nature communications 13:6090 PubMed36241664

2022

Solving the MCM paradox by visualizing the scaffold of CMG helicase at active replisomes.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Polasek-Sedlackova,Thomas C R Miller,Jana Krejci,Maj-Britt Rask,Jiri Lukas
View all publications
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