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AB109436

Anti-TIM50 抗体 [EPR5785]

Anti-TIM50 antibody [EPR5785]

5

(2 Reviews)

|

(5 Publications)

Rabbit Recombinant Monoclonal TIM50 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

別名を表示する

TIM50, PRO1512, TIMM50, Mitochondrial import inner membrane translocase subunit TIM50

2 Images
Western blot - Anti-TIM50 antibody [EPR5785] (AB109436)
  • WB

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Western blot - Anti-TIM50 antibody [EPR5785] (AB109436)

All lanes:

Western blot - Anti-TIM50 antibody [EPR5785] (ab109436) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 10 µg

Lane 3:

Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Predicted band size: 40 kDa

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OI-RD Scanning - Anti-TIM50 antibody [EPR5785] (AB109436)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TIM50 antibody [EPR5785] (AB109436)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-TIM50 antibody [EPR5785] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5785

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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製品の詳細

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIM50 also known as Translocase of Inner Mitochondrial Membrane 50 is a protein weighing approximately 50 kDa. This protein plays an important mechanical role in the mitochondrial presequence translocase functioning as a receptor and import channel within the inner mitochondrial membrane. TIM50 is expressed predominantly within the inner membranes of mitochondria in eukaryotic cells.
Biological function summary

TIM50 facilitates the import of presequence-containing proteins from the cytosol into the mitochondrial matrix acting as part of the TIM23 complex. This complex orchestrates the translocation of proteins across the inner mitochondrial membrane. By interacting with presequences of nuclear-encoded mitochondrial proteins TIM50 ensures proper import and processing which is important for mitochondrial function and biogenesis.

Pathways

TIM50 is critical in the mitochondrial protein import pathway which is essential for mitochondrial respiration and energy production. TIM50 interacts with other proteins such as TIM23 and TIM17 to form the translocase of the inner membrane 23 (TIM23) complex. TIM50 also links to the mitochondrial unfolded protein response (UPR) where it may influence the regulation of proteostasis within mitochondria alongside proteins like HSP70.

Mutations and dysregulation of TIM50 have connections to mitochondrial diseases which encompass a range of disorders due to mitochondrial dysfunction. Additionally TIM50 may affect neurodegenerative diseases where mitochondrial impairment plays a role. Alterations in TIM50 influencing mitochondrial function might correlate with proteins like cytochrome c which can contribute to apoptotic pathways and cell death in these diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo.. Isoform 2. May participate in the release of snRNPs and SMN from the Cajal body.
See full target information TIMM50
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:935710 PubMed36451821

2022

The mitochondrial gene-CMPK2 functions as a rheostat for macrophage homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Prabhakar Arumugam,Meghna Chauhan,Thejaswitha Rajeev,Rahul Chakraborty,Kanika Bisht,Mahima Madan,Deepthi Shankaran,Sivaprakash Ramalingam,Sheetal Gandotra,Vivek Rao

The Journal of experimental medicine 219: PubMed35616614

2022

MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Katsouda,Dimitrios Valakos,Vasilios S Dionellis,Sofia-Iris Bibli,Ioannis Akoumianakis,Sevasti Karaliota,Karim Zuhra,Ingrid Fleming,Noriyuki Nagahara,Sophia Havaki,Vassilis G Gorgoulis,Dimitris Thanos,Charalambos Antoniades,Csaba Szabo,Andreas Papapetropoulos

The journal of physiological sciences : JPS 69:359-373 PubMed30570704

2018

Regulation of mitochondrial iron homeostasis by sideroflexin 2.

Applications

Unspecified application

Species

Unspecified reactive species

Ei Ei Mon,Fan-Yan Wei,Raja Norazireen Raja Ahmad,Takahiro Yamamoto,Toshiro Moroishi,Kazuhito Tomizawa

Human molecular genetics 27:3881-3900 PubMed30084972

2018

CHCHD2 accumulates in distressed mitochondria and facilitates oligomerization of CHCHD10.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Huang,Beverly P Wu,Diana Nguyen,Yi-Ting Liu,Melika Marani,Jürgen Hench,Paule Bénit,Vera Kozjak-Pavlovic,Pierre Rustin,Stephan Frank,Derek P Narendra

Nature neuroscience 17:822-31 PubMed24836077

2014

Inhibition of mitochondrial protein import by mutant huntingtin.

Applications

Unspecified application

Species

Mouse

Hiroko Yano,Sergei V Baranov,Oxana V Baranova,Jinho Kim,Yanchun Pan,Svitlana Yablonska,Diane L Carlisle,Robert J Ferrante,Albert H Kim,Robert M Friedlander
View all publications
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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。
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