AB167423

Anti-TIM22 抗体 [EPR9973]

Name: Anti-TIM22 抗体 [EPR9973] (ab167423) | Abcam
Brand: Abcam Limited
SKU: AB167423
Price: 45000 JPY
Availability: InStock
Rating: 4 (1 reviews)

Anti-TIM22 antibody [EPR9973]

RabMAb
Recombinant
詳細を見る

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal TIM22 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

別名を表示する

TEX4, TIM22, TIMM22, Mitochondrial import inner membrane translocase subunit Tim22, Testis-expressed protein 4

1 Images

Unknown

Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

All lanes:

Western blot - Anti-TIM22 antibody [EPR9973] (ab167423) at 1/1000 dilution

Lane 1:

A673 cell lysate at 10 µg

Lane 2:

T47D cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Lane 4:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 20 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR9973

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製度

Tissue culture supernatant

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The TIM22 protein also known as Translocase of Inner Mitochondrial Membrane 22 has a molecular mass of approximately 20 kilodaltons. It plays an important mechanical role in the import and insertion of membrane proteins into the mitochondrial inner membrane. TIM22 locates itself in the mitochondrial inner membrane where it functions as part of a larger translocase complex.

Biological function summary

TIM22 interacts with several mitochondria-rich cellular environments to facilitate the integration of polytopic proteins. As part of the TIM22 complex it integrates endeavored proteins into the mitochondrial inner membrane. This integration is essential for the optimal function of the mitochondria. It interacts closely with other components including TIM54 and TIM18 which assist in its function within the complex.

Pathways

The TIM22 complex activities heavily contribute to the mitochondrial import pathways. It remains connected with the translocase of outer mitochondrial membrane (TOM) complex as it receives incoming proteins. TIM22 connects with TOM40 and small TIM such as TIM9 and TIM10 during the import and sorting processes. This integration ensures that proteins achieve the correct membrane insertion needed for mitochondrial function.

TIM22-related dysfunction can lead to mitochondrial-related disorders such as mitochondrial myopathy. This happens when the protein integration into the mitochondrial membrane faces disruption. Additionally deficiencies or mutations in TIM22 have links to neurodegenerative disorders where proper mitochondrial function is compromised. The interaction with other members of the mitochondrial membrane translocase complexes like TIM54 and TIM18 is important in these disorder contexts.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps (By similarity).

See full target information TIMM22

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 34:108869 PubMed33730581

2021

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle Grace Acoba,Ebru S Selen Alpergin,Santosh Renuse,Lucía Fernández-Del-Río,Ya-Wen Lu,Oleh Khalimonchuk,Catherine F Clarke,Akhilesh Pandey,Michael J Wolfgang,Steven M Claypool

Journal of cellular biochemistry 122:626-638 PubMed33522619

2021

The transcriptional regulator Zfat is essential for maintenance and differentiation of the adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Ishikura,Masayoshi Nagai,Toshiyuki Tsunoda,Kensuke Nishi,Yoko Tanaka,Midori Koyanagi,Senji Shirasawa

Scientific reports 9:2822 PubMed30808977

2019

Mieap-induced accumulation of lysosomes within mitochondria (MALM) regulates gastric cancer cell invasion under hypoxia by suppressing reactive oxygen species accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Keiichiro Okuyama,Yoshihiko Kitajima,Noriyuki Egawa,Hiroshi Kitagawa,Kotaro Ito,Shinichi Aishima,Kazuyoshi Yanagihara,Tomokazu Tanaka,Hirokazu Noshiro

Stem cell reviews and reports 15:427-438 PubMed30338499

2018

Metabolic Syndrome Modulates Protein Import into the Mitochondria of Porcine Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Arash Aghajani Nargesi,Xiang-Yang Zhu,LaTonya J Hickson,Sabena M Conley,Andre J van Wijnen,Lilach O Lerman,Alfonso Eirin

American journal of human genetics 99:860-876 PubMed27693233

2016

Recurrent De Novo Dominant Mutations in SLC25A4 Cause Severe Early-Onset Mitochondrial Disease and Loss of Mitochondrial DNA Copy Number.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle Thompson,Homa Majd,Cristina Dallabona,Karit Reinson,Martin S King,Charlotte L Alston,Langping He,Tiziana Lodi,Simon A Jones,Aviva Fattal-Valevski,Nitay D Fraenkel,Ann Saada,Alon Haham,Pirjo Isohanni,Roshni Vara,Inês A Barbosa,Michael A Simpson,Charu Deshpande,Sanna Puusepp,Penelope E Bonnen,Richard J Rodenburg,Anu Suomalainen,Katrin Õunap,Orly Elpeleg,Ileana Ferrero,Robert McFarland,Edmund R S Kunji,Robert W Taylor

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TIM22 抗体 [EPR9973]

Anti-TIM22 antibody [EPR9973]

Western blot - Anti-TIM22 antibody [EPR9973] (AB167423)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製度

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (5)

組合せ製品

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise