Anti-TIM 4 抗体 (ab47637)
Key features and details
- Rabbit polyclonal to TIM 4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-TIM 4 antibody
TIM 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIM 4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human TIM 4 (internal sequence). A 16 aminoacid synthetic peptide from near the center of human TIM 4
Database link: Q96H15 -
ポジティブ･コントロール
- Jurkat whole cell lysate (ab7899)
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47637の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
|
|
| ICC/IF |
Use a concentration of 10 - 20 µg/ml.
|
|
| IHC-P |
Use a concentration of 2 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa. |
|
ICC/IF
Use a concentration of 10 - 20 µg/ml. |
|
IHC-P
Use a concentration of 2 µg/ml. |
ターゲット情報
-
機能
Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. -
配列類似性
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Recognizes phosphatidyl serine via its immunoglobulin domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 91937 Human
- Entrez Gene: 276891 Mouse
- Omim: 610096 Human
- SwissProt: Q96H15 Human
- SwissProt: Q6U7R4 Mouse
- Unigene: 334907 Human
- Unigene: 69002 Mouse
-
別名
- SMUCKLER antibody
- Spleen, mucin-containing, knockout of lymphotoxin protein antibody
- T cell immunoglobulin and mucin domain containing protein 4 antibody
see all
画像
-
Western blot - Anti-TIM 4 antibody (ab47637)Western Blot of RAW264.7 cell lysate labeling TIM 4 with Anti-TIM 4 antibody (ab47637) at 2µg/ml in (1) the absence and (2) the presence of blocking peptide.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM 4 antibody (ab47637)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse liver tissue staining TIM 4 with ab47637 at 2µg/ml -
Western blot - Anti-TIM 4 antibody (ab47637)Lane 1 : Anti-TIM 4 antibody (ab47637) at 1 µg/ml
Lane 2 : Anti-TIM 4 antibody (ab47637) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 42 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa, 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (ab47637)ab47637 at 10µg/ml staining TIM 4 in Jurkat cells by ICC/IF
-
Immunocytochemistry/ Immunofluorescence - Anti-TIM 4 antibody (ab47637)Immunofluorescence of TIM-4 in Jurkat cells using ab47637 at 20 ug/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab47637 は 9 報の論文で使用されています。
- Wang Y et al. Tim-4 reprograms cholesterol metabolism to suppress antiviral innate immunity by disturbing the Insig1-SCAP interaction in macrophages. Cell Rep 41:111738 (2022). PubMed: 36450259
- Asare PF et al. Inhibition of LC3-associated phagocytosis in COPD and in response to cigarette smoke. Ther Adv Respir Dis 15:17534666211039769 (2021). PubMed: 34852704
- Zheng L et al. Inhibition of TIM-4 protects against cerebral ischaemia-reperfusion injury. J Cell Mol Med 24:1276-1285 (2020). PubMed: 31774937
- Zhang Y et al. Hepatic Ischemic Preconditioning Alleviates Ischemia-Reperfusion Injury by Decreasing TIM4 Expression. Int J Biol Sci 14:1186-1195 (2018). PubMed: 30123068
- Mukherjee S et al. Boosting efferocytosis in alveolar space using BCG vaccine to protect host against influenza pneumonia. PLoS One 12:e0180143 (2017). WB ; Mouse . PubMed: 28686604
- Park SY et al. Stabilin-2 modulates the efficiency of myoblast fusion during myogenic differentiation and muscle regeneration. Nat Commun 7:10871 (2016). PubMed: 26972991
- Zhang Q et al. TIM-4 promotes the growth of non-small-cell lung cancer in a RGD motif-dependent manner. Br J Cancer 113:1484-92 (2015). PubMed: 26512878
- Kim HS et al. T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production. J Immunol 184:4095-106 (2010). Flow Cyt ; Mouse . PubMed: 20220086
- Xu L et al. T cell immunoglobulin- and mucin-domain-containing molecule-4 attenuates concanavalin A-induced hepatitis by regulating macrophage. J Leukoc Biol 88:329-36 (2010). WB ; Mouse . PubMed: 20453106