Anti-TIM 1 抗体 [mAbcam75295] (ab75295)
![Western blot - Anti-TIM 1 antibody [mAbcam75295] (ab75295)](https://www.abcam.co.jp/ps/products/75/ab75295/Images/TIM-1-Primary-antibodies-ab75295-2.jpg "Western blot - Anti-TIM 1 antibody [mAbcam75295] (ab75295)")
Key features and details
- Mouse monoclonal [mAbcam75295] to TIM 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TIM 1 antibody [mAbcam75295]
TIM 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mAbcam75295] to TIM 1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TIM 1 aa 300 to the C-terminus (C terminal).
(Peptide available asab97979) -
ポジティブ･コントロール
- This antibody gave a positive signal in the human testis and brain tissue lysates.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
mAbcam75295 -
ミエローマ
Sp2/0-Ag14 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
|
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
組織特異性
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
配列類似性
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26762 Human
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- Unigene: 129711 Human
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別名
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
画像
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Western blot - Anti-TIM 1 antibody [mAbcam75295] (ab75295)All lanes : Anti-TIM 1 antibody [mAbcam75295] (ab75295) at 10 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
The band at approximately 50-kDa is comparable to the molecular weight seen with other antibodies to TIM 1. TIM 1 contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab75295 は論文での使用が確認できていません。