Anti-TIM 1 抗体 [9E1] (ab302932)
![Western blot - Anti-HAVCR1 antibody [9E1] (AB302932)](https://www.abcam.co.jp/ps/products/302/ab302932/Images/AB302932-489173-anti-havcr1-antibody-9e1-wb-raw264-7--293t-hepg2-and-nih3t3.jpg "Western blot - Anti-HAVCR1 antibody [9E1] (AB302932)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [9E1] to TIM 1
- Suitable for: WB
- Reacts with: Mouse, Human
製品の概要
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製品名
Anti-TIM 1 antibody [9E1]
TIM 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [9E1] to TIM 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: RAW264.7, 293T, HepG2, and NIH/3T3 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
9E1 -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302932の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
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| 特記事項 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa). |
ターゲット情報
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機能
May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4 (By similarity). In case of human hepatitis A virus (HHAV) infection, functions as a cell-surface receptor for the virus. May play a role in kidney injury and repair. -
組織特異性
Widely expressed, with highest levels in kidney and testis. Expressed by activated CD4+ T-cells during the development of helper T-cells responses. -
配列類似性
Belongs to the immunoglobulin superfamily. TIM family.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 26762 Human
- Entrez Gene: 171283 Mouse
- Omim: 606518 Human
- SwissProt: Q96D42 Human
- SwissProt: Q5QNS5 Mouse
- Unigene: 129711 Human
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別名
- CD365 antibody
- HAVCR 1 antibody
- HAVCR antibody
see all
画像
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Western blot - Anti-HAVCR1 antibody [9E1] (AB302932)All lanes : Anti-TIM 1 antibody [9E1] (ab302932) at 1/1000 dilution
Lane 1 : RAW264.7 (murine leukemia virus-induced tumor cell) whole cell
lysate
Lane 2 : 293T (Human embryonic kidney cell line) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab302932 は 1 報の論文で使用されています。
- Li L et al. Knockdown of ELF4 aggravates renal injury in ischemia/reperfusion mice through promotion of pyroptosis, inflammation, oxidative stress, and endoplasmic reticulum stress. BMC Mol Cell Biol 24:22 (2023). PubMed: 37474923