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リコンビナントRabMAb

Anti-TIL/TLR1 抗体 [EPR2075] (ab68158)

Submit a review Submit a question References (1)
Western blot - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
  • Flow Cytometry - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
  • Immunoprecipitation - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
  • Anti-TIL/TLR1 antibody [EPR2075] (ab68158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2075] to TIL/TLR1
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TIL/TLR1 antibody [EPR2075]
    TIL/TLR1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2075] to TIL/TLR1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Flow Cytmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human TIL/TLR1 aa 150-250 (extracellular). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • WB: Daudi and Ramos cell lysates. Flow Cyt: Daudi cells. IP: Ramos cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab68158の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
IP
Flow Cyt
特記事項
追加情報
Flow Cyt: 1/60.
IP: 1/40.
WB: 1/1000 - 1/2000. Detects a band of approximately 90 and 125 kDa (predicted molecular weight: 90 kDa).

Is unsuitable for ICC or IHC-P.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 機能

    Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

  • 組織特異性

    Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 12 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • 細胞内局在

    Cell membrane. Cytoplasmic vesicle > phagosome membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD281 antibody
      • CD281 antigen antibody
      • KIAA0012 antibody
      • LPRS5 antibody
      • rsc786 antibody
      • TIL antibody
      • TIL. LPRS5 antibody
      • Tlr1 antibody
      • TLR1_HUMAN antibody
      • Toll (Drosophila) homolog antibody
      • Toll like receptor 1 antibody
      • Toll-like receptor 1 antibody
      • Toll/interleukin-1 receptor-like protein antibody
      see all

    画像

    • Western blot - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
      Western blot - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
      All lanes : Anti-TIL/TLR1 antibody [EPR2075] (ab68158) at 1/1000 dilution

      Lane 1 : Daudi cell lysate
      Lane 2 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 90 kDa
      Observed band size: 90 kDa
      Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.

    • Flow Cytometry - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
      Flow Cytometry - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)

      Flow cytometric analysis of TIL/TLR1 in Daudi cells using ab68158 at 1/60 dilution (pink) or a rabbit IgG (green).

    • Immunoprecipitation - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
      Immunoprecipitation - Anti-TIL/TLR1 antibody [EPR2075] (ab68158)

      TIL/TLR1 was immunoprecipitated from 0.35 mg Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg with ab68158 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 µg

      Lane 2: ab68158 IP in Ramos whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab68158 in Ramos whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

       

    • Anti-TIL/TLR1 antibody [EPR2075] (ab68158)
      Anti-TIL/TLR1 antibody [EPR2075] (ab68158)

    参考文献 (1)

    ab68158 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab68158 は 1 報の論文で使用されています。

    • Galoian K  et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). PubMed: 29138803

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