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AB37068

Anti-TIL/TLR1 抗体

Anti-TIL/TLR1 antibody

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(3 Publications)

Rabbit Polyclonal TIL/TLR1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TLR1.

別名を表示する

CD281, KIAA0012, TLR1, Toll-like receptor 1, Toll/interleukin-1 receptor-like protein, TIL

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (AB37068)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (AB37068)

Immunofluorescence of TLR1 in Mouse Spleen cells using ab37068 at 20 ug/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIL/TLR1 antibody (AB37068)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIL/TLR1 antibody (AB37068)

ab37068 at 10μg/ml staining TIL/TLR1 in mouse spleen tissue by IHC

Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (AB37068)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TIL/TLR1 antibody (AB37068)

ICC/IF image of ab37068 stained RAW246.7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37068, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Western blot - Anti-TIL/TLR1 antibody (AB37068)
  • WB

Unknown

Western blot - Anti-TIL/TLR1 antibody (AB37068)

Lane 1:

Western blot - Anti-TIL/TLR1 antibody (ab37068) at 1 µg/mL

Lane 2:

Western blot - Anti-TIL/TLR1 antibody (ab37068) at 2 µg/mL

Lane 3:

Western blot - Anti-TIL/TLR1 antibody (ab37068) at 4 µg/mL

All lanes:

Mouse spleen lysate at 15 µg

Predicted band size: 90 kDa

Observed band size: 75 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse

アプリケーション

WB, ICC/IF, IHC-P

applications

免疫原

Synthetic Peptide within Human TLR1. The exact immunogen used to generate this antibody is proprietary information.

Q15399

特異性

ab37068 recognises Toll-like receptor 1 (TIL/TLR1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
Purified by ion exchange chromatography
バッファー組成
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Toll-like receptor 1 (TLR1) also known as TIL plays a significant role in the immune system. It is a pattern recognition receptor that detects pathogen-associated molecular patterns (PAMPs). TLR1 has a molecular weight of approximately 90 kilodaltons. It is expressed on the surface of various immune cells including monocytes macrophages and dendritic cells. TLR1 recognizes triacylated lipopeptides in combination with TLR2 forming a heterodimer that is essential for signaling. This interaction enhances the immune response against microbial infections.
Biological function summary

TLR1 contributes to the immune response by participating in the detection of bacterial lipoproteins. This receptor is a part of the Toll-like receptor family which plays an essential role in innate immunity. TLR1 often in complex with TLR2 helps in recognizing and responding to the structural motifs of microbial pathogens. These interactions lead to the activation of downstream signaling pathways triggering the production of cytokines and other mediators essential for inflammatory responses.

Pathways

TLR1 is involved in the Toll-like receptor signaling pathway and the NF-kB signaling pathway. In the Toll-like receptor pathway TLR1-TLR2 heterodimers signal through adaptor proteins like MyD88 which leads to the activation of transcription factors such as NF-kB. This mechanism regulates the expression of genes involved in immune and inflammatory responses. TLR1 coordinates with TLR2 not just in recognition of ligands but also in the signaling to produce a coordinated immune response enhancing the cell's ability to deal with infections.

TLR1 plays a role in conditions involving immune dysregulation such as tuberculosis and sepsis. Genetic variations in TLR1 can impact susceptibility to these diseases by altering the receptor's ability to recognize pathogenic components. TLR1's interaction with TLR2 influences the inflammatory response and may contribute to inappropriate immune activation seen in sepsis. These receptors' roles in inflammation and pathogen recognition highlight their importance in understanding the development and progression of immune-related diseases.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed : 21078852). Forms the activation cluster TLR2 : TLR1 : CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed : 16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
See full target information TLR1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7466 PubMed36463242

2022

Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Junmeng Zhu,Yaohua Ke,Qin Liu,Ju Yang,Fangcen Liu,Ruihan Xu,Hang Zhou,Aoxing Chen,Jie Xiao,Fanyan Meng,Lixia Yu,Rutian Li,Jia Wei,Baorui Liu

International journal of molecular medicine 46:239-251 PubMed32377717

2020

Puerarin improves graft bone defect through microRNA‑155‑3p‑mediated p53/TNF‑α/STAT1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhou,Hongyu Lian,Kexin Liu,Deli Wang,Xuelian Xiu,Zhang Sun

The Journal of infectious diseases 200:546-54 PubMed19586419

2009

Age-associated inflammation and toll-like receptor dysfunction prime the lungs for pneumococcal pneumonia.

Applications

WB

Species

Mouse

Ernesto Hinojosa,Angela R Boyd,Carlos J Orihuela
View all publications
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組合せ製品

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