Anti-TIGIT 抗体 [4D4] (ab289547)
![Flow Cytometry - Anti-TIGIT antibody [4D4] (ab289547)](https://www.abcam.co.jp/ps/products/289/ab289547/Images/ab289547-487105-TIGIT4D4.jpg "Flow Cytometry - Anti-TIGIT antibody [4D4] (ab289547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Armenian hamster monoclonal [4D4] to TIGIT
- Suitable for: Flow Cyt, Indirect ELISA
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-TIGIT antibody [4D4]
TIGIT 一次抗体 製品一覧 -
製品の詳細
Armenian hamster monoclonal [4D4] to TIGIT -
由来種
Armenian hamster -
アプリケーション
適用あり: Flow Cyt, Indirect ELISAmore details
適用なし: IHC-P -
種交差性
交差種: Mouse
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: Mouse splenocytes treated with 2.5ug/ml Concanavalin A (ConA) for 72h. Indirect ELISA: Mouse TIGIT protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
4D4 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab289547の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
1/1000.
|
|
| Indirect ELISA |
Use a concentration of 0.001 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt
1/1000. |
|
Indirect ELISA
Use a concentration of 0.001 µg/ml. |
ターゲット情報
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機能
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. -
組織特異性
Expressed at low levels on peripheral memory and regulatory CD4+ T cells and NK cells and is up-regulated following activation of these cells (at protein level). -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 100043314 Mouse
- SwissProt: P86176 Mouse
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別名
- DKFZp667A205 antibody
- ENSMUSG00000071552 antibody
- FLJ39873 antibody
see all
画像
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Flow Cytometry - Anti-TIGIT antibody [4D4] (ab289547)
Flow cytometric analysis of mouse splenocytes treated with 2.5 μg/ml Concanavalin A (ConA) for 72h labelling TIGIT with ab289547 at 1/1000 dilution (Right) compared with a American Hamster monoclonal IgG isotype control (Left). A Goat anti American Hamster IgG at 1/500 dilution was used as the secondary antibody.
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![Indirect ELISA - Anti-TIGIT antibody [4D4] (ab289547)](https://www.abcam.co.jp/ps/products/289/ab289547/Images/ab289547-487106-4D4YS092301PSRtemplate.png)
Indirect ELISA - Anti-TIGIT antibody [4D4] (ab289547)Indirect ELISA antibody dose-response curve using ab289547 between 0-2000 ng/mL. Antigen concentration of 1000 ng/mL. An alkaline phosphatase-conjugated goat anti-American Hamster IgG (H+L) at 1/1000 dilution was used as the secondary antibody.
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![Anti-TIGIT antibody [4D4] (ab289547)](https://www.abcam.co.jp/ps/products/289/ab289547/Images/ab289547-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-TIGIT antibody [4D4] (ab289547)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab289547 は論文での使用が確認できていません。