Anti-TIGAR 抗体 (ab37910)
Key features and details
- Rabbit polyclonal to TIGAR
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TIGAR antibody
TIGAR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TIGAR -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TIGAR aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab37909) -
ポジティブ･コントロール
- Recombinant Human TIGAR protein (ab115509) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK 293 (Human embryonic kidney cell line) HepG2 (Human hepatocellular liver carcinoma cell line) MCF-7 (Human breast adenocarcinoma cell line)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab37910の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| WB | (1) |
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/250. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa). |
ターゲット情報
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機能
Probable fructose-biphosphatase. Lowers cellular levels of fructose 2,6-bisphosphate. Protects cells against reactive oxygen species and against apoptosis induced by p53/TP53. -
配列類似性
Belongs to the phosphoglycerate mutase family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. - Information by UniProt
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参照データベース
- Entrez Gene: 57103 Human
- Omim: 610775 Human
- SwissProt: Q9NQ88 Human
- Unigene: 504545 Human
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別名
- 6-bisphosphatase TIGAR antibody
- C12ORF5 antibody
- chromosome 12 open reading frame 5 antibody
see all
画像
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Western blot - Anti-TIGAR antibody (ab37910)All lanes : Anti-TIGAR antibody (ab37910) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 : A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : Hep G2 whole cell lysate (ab7900)
Lane 6 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 30 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-TIGAR antibody (ab37910)ICC/IF image of ab37910 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37910 at 1µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab37910 は 27 報の論文で使用されています。
- Britt EC et al. Switching to the cyclic pentose phosphate pathway powers the oxidative burst in activated neutrophils. Nat Metab 4:389-403 (2022). PubMed: 35347316
- Wang H et al. Nuclear TIGAR mediates an epigenetic and metabolic autoregulatory loop via NRF2 in cancer therapeutic resistance. Acta Pharm Sin B 12:1871-1884 (2022). PubMed: 35847493
- Yin N et al. IDH1-R132H mutation radiosensitizes U87MG glioma cells via epigenetic downregulation of TIGAR. Oncol Lett 19:1322-1330 (2020). PubMed: 31966064
- Keller F et al. A Scaffold-Free 3-D Co-Culture Mimics the Major Features of the Reverse Warburg Effect In Vitro. Cells 9:N/A (2020). PubMed: 32823793
- Domingo-Vidal M et al. Cigarette Smoke Induces Metabolic Reprogramming of the Tumor Stroma in Head and Neck Squamous Cell Carcinoma. Mol Cancer Res 17:1893-1909 (2019). PubMed: 31239287