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リコンビナントRabMAb

Anti-TIE2 抗体 [EPR25622-92] - BSA and Azide free (Capture) (ab300687)

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Sandwich ELISA - Anti-TIE2 antibody [EPR25622-92] - BSA and Azide free (Capture) (ab300687)
  • Anti-TIE2 [EPR25622-92] - BSA and Azide free (Capture) (AB300687)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR25622-92] to TIE2 - BSA and Azide free (Capture)
  • Suitable for: Sandwich ELISA
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-TIE2 antibody [EPR25622-92] - BSA and Azide free (Capture)
    TIE2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR25622-92] to TIE2 - BSA and Azide free (Capture)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 種交差性

    交差種: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 特記事項

    ab300687 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify mouse, rat TIE2. The recommended pair for sandwich ELISA is:
         Capture: ab300687, mouse TIE2 Capture Antibody (unconjugated)
         Detector: ab300688, mouse TIE2 Detector Antibody (unconjugated)
    The reference range value is 31.25-2000pg/ml

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab300687の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25622-80] to TIE2 - BSA and Azide free (Detector) (ab300688).
特記事項
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR25622-80] to TIE2 - BSA and Azide free (Detector) (ab300688).

ターゲット情報

  • 機能

    Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.

  • 組織特異性

    Detected in umbilical vein endothelial cells. Proteolytic processing gives rise to a soluble extracellular domain that is detected in blood plasma (at protein level). Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.

  • 関連疾患

    Dominantly inherited venous malformations
    May play a role in a range of diseases with a vascular component, including neovascularization of tumors, psoriasis and inflammation.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • ドメイン

    The soluble extracellular domain is functionally active in angiopoietin binding and can modulate the activity of the membrane-bound form by competing for angiopoietins.

  • 翻訳後修飾

    Proteolytic processing leads to the shedding of the extracellular domain (soluble TIE-2 alias sTIE-2).
    Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner, where Tyr-992 in the kinase activation loop is phosphorylated first, followed by autophosphorylation at Tyr-1108 and at additional tyrosine residues. ANGPT1-induced phosphorylation is impaired during hypoxia, due to increased expression of ANGPT2. Phosphorylation is important for interaction with GRB14, PIK3R1 and PTPN11. Phosphorylation at Tyr-1102 is important for interaction with SHC1, GRB2 and GRB7. Phosphorylation at Tyr-1108 is important for interaction with DOK2 and for coupling to downstream signal transduction pathways in endothelial cells. Dephosphorylated by PTPRB.
    Ubiquitinated. The phosphorylated receptor is ubiquitinated and internalized, leading to its degradation.

  • 細胞内局在

    Cell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Angiopoietin 1 receptor antibody
      • Angiopoietin-1 receptor antibody
      • CD202b antibody
      • CD202b antigen antibody
      • Endothelial tyrosine kinase antibody
      • Endothelium specific receptor tyrosine kinase 2 antibody
      • hTIE 2 antibody
      • hTIE2 antibody
      • Hyk antibody
      • p140 TEK antibody
      • Soluble TIE2 variant 1 antibody
      • Soluble TIE2 variant 2 antibody
      • Tek antibody
      • tek tyrosine kinase antibody
      • TEK tyrosine kinase endothelial antibody
      • tek tyrosine kinase, endothelial antibody
      • TIE 2 antibody
      • TIE2 antibody
      • TIE2_HUMAN antibody
      • Tunica interna endothelial cell kinase antibody
      • Tyrosine kinase with Ig and EGF homology domains 2 antibody
      • Tyrosine kinase with Ig and EGF homology domains-2 antibody
      • Tyrosine protein kinase receptor TEK antibody
      • Tyrosine protein kinase receptor TIE 2 antibody
      • Tyrosine-protein kinase receptor TEK antibody
      • Tyrosine-protein kinase receptor TIE-2 antibody
      • Venous malformations multiple cutaneous and mucosal antibody
      • VMCM 1 antibody
      • VMCM antibody
      • VMCM1 antibody
      see all

    画像

    • Sandwich ELISA - Anti-TIE2 antibody [EPR25622-92] - BSA and Azide free (Capture) (ab300687)
      Sandwich ELISA - Anti-TIE2 antibody [EPR25622-92] - BSA and Azide free (Capture) (ab300687)
      Sandwich ELISA of ab300686 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL
    • Anti-TIE2 [EPR25622-92] - BSA and Azide free (Capture) (AB300687)
      Anti-TIE2 [EPR25622-92] - BSA and Azide free (Capture) (AB300687)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab300687 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab300687 は論文での使用が確認できていません。

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