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リコンビナントRabMAb

Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) 抗体 [EPR1053(N)(B)] (ab151704)

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Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
  • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)
  • Suitable for: WB, ICC/IF, Dot blot
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)]

  • 製品の詳細

    Rabbit monoclonal [EPR1053(N)(B)] to TIE1 (phospho Y1007) + TIE2 (phospho Y992)

  • 由来種

    Rabbit

  • 特異性

    ab151704 only detects TIE1 phosphorylated at tyrosine 1007 and TIE2 phosphorylated at tyrosine 992.

  • アプリケーション

    適用あり: WB, ICC/IF, Dot blotmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HUVEC cell lysate treated with pervanadate. ICC/IF: HUVEC cells treated with pervanadate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab151704の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
ICC/IF
1/250 - 1/500.
Dot blot
1/1000.
特記事項
WB
1/10000 - 1/50000. Predicted molecular weight: 125 kDa.
ICC/IF
1/250 - 1/500.
Dot blot
1/1000.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 細胞内局在

    TIE1: Cell membrane. TIE2: Cell membrane. Cell junction. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Secreted. Recruited to cell-cell contacts in quiescent endothelial cells. Colocalizes with the actin cytoskeleton and at actin stress fibers during cell spreading. Recruited to the lower surface of migrating cells, especially the rear end of the cell. Proteolytic processing gives rise to a soluble extracellular domain that is secreted.

  • 参照データベース

    画像

    • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : HUVEC cell lysate treated with pervanadate
      Lane 2 : HUVEC cell lysate treated with pervanadate with TIE2 (phospho Y992) peptide
      Lane 3 : HUVEC cell lysate treated with pervanadate with TIE2 unmodified peptide
      Lane 4 : HUVEC cell lysate treated with pervanadate with TIE1 (phospho Y1007) peptide
      Lane 5 : HUVEC cell lysate treated with pervanadate with TIE1 unmodified peptide

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000000 dilution (HRP goat anti-rabbit IgG (H+L))

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 5 seconds


      Blocking buffer: 5% BSA/TBST
      Dilution buffer: 5% BSA /TBST for primary antibody, 5% NFDM/TBST for secondary antibody

    • Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Immunocytochemistry/ Immunofluorescence - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

      Immunocytochemistry/Immunofluorescence analysis of HUVEC () cells labelling TIE2 + TIE1 (phospho Y992 + Y1007) with ab151704 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 anti-Tubulin (mouse mAb) at a dilution of 1/500 and ab150120 AlexaFluor®594 Goat anti-Mouse secondary at 1/1000. Nuclei were counterstained with DAPI (blue).

      Confocal image showing increased cytoplasmic staining after PER (Pervanadate, 1mM, 30min) treatment on HUVEC cells. The LP treatment decreased the PER induced cytoplasmic staining. 

    • Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Western blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      All lanes : Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704) at 1/100000 dilution

      Lane 1 : Untreated HUVEC whole cell lysates
      Lane 2 : HUVEC treated with Pervanadate whole cell lysates
      Lane 3 : HUVEC treated with Pervanadate whole cell lysates, then the membrane was incubated with phosphatase.

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 125 kDa
      Observed band size: 125 kDa


      Exposure time: 10 seconds


      Blocking/Diluting buffer 5% NFDM/TBST

    • Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Dot Blot - Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

      Dot blot analysis of TIE2 (pY992) phospho peptide (lane 1) and TIE2 non-phospho peptide (lane 2) labelling TIE2 (phospho Y992) with ab151704 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 10 seconds.

    • Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)
      Anti-TIE1 (phospho Y1007) + TIE2 (phospho Y992) antibody [EPR1053(N)(B)] (ab151704)

    参考文献 (4)

    ab151704 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab151704 は 4 報の論文で使用されています。

    • Chen S  et al. Role of UDP-glucose ceramide glucosyltransferase in venous malformation. Front Cell Dev Biol 11:1178045 (2023). PubMed: 37274734
    • Zhou H  et al. Glycation of Tie-2 Inhibits Angiopoietin-1 Signaling Activation and Angiopoietin-1-Induced Angiogenesis. Int J Mol Sci 23:N/A (2022). PubMed: 35806141
    • Koya Y  et al. An engineered tetra-valent antibody fully activates the Tie2 receptor with comparable potency to its natural ligand angiopoietin-1. Sci Rep 11:14021 (2021). PubMed: 34234265
    • Messerschmidt VL  et al. Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules. Front Cardiovasc Med 8:707897 (2021). PubMed: 34651022

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