Anti-TIE1 抗体 [8C9] (ab27851)
Key features and details
- Mouse monoclonal [8C9] to TIE1
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-TIE1 antibody [8C9]
TIE1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8C9] to TIE1 -
由来種
Mouse -
アプリケーション
適用あり: WB, Flow Cytmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human TIE1. Recombinant human soluble extracellular TIE1.
Database link: P35590 -
ポジティブ・コントロール
- Flow Cyt: K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate WB: Recombinant human soluble TIE1
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
This antibody was purified from cell culture supernatant by chromatography. -
ポリ/モノ
モノクローナル -
クローン名
8C9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab27851の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use a concentration of 200000 - 500000 µg/ml. Predicted molecular weight: 125 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. -
組織特異性
Specifically expressed in developing vascular endothelial cells. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated on tyrosine residues in response to ANGPT1, most likely by TEK/TIE2. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7075 Human
- Omim: 600222 Human
- SwissProt: P35590 Human
- Unigene: 78824 Human
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別名
- JKT 14 antibody
- JTK14 antibody
- TIE antibody
see all
画像
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Overlay histogram showing K562 cells stained with ab27851 (red line). The cells were fixed with 80% methanol (5 min) and then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27851, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
All lanes : Anti-TIE1 antibody [8C9] (ab27851)
Lane 1 : Recombinant human soluble TIE1
Lane 2 : Recombinant human soluble TIE2
Lane 3 : Recombinant mouse soluble TIE1
Lane 4 : Recombinant mouse soluble TIE2
Predicted band size: 125 kDa
データシートおよび資料
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Datasheet download
参考文献 (5)
ab27851 は 5 報の論文で使用されています。
- Zhang J et al. Melatonin Promotes Heterotopic Ossification Through Regulation of Endothelial-Mesenchymal Transition in Injured Achilles Tendons in Rats. Front Cell Dev Biol 9:629274 (2021). PubMed: 33644068
- Liu T et al. MiR-4729 regulates TIE1 mRNA m6A modification and angiogenesis in hemorrhoids by targeting METTL14. Ann Transl Med 9:232 (2021). PubMed: 33708859
- Hassanpour M et al. Resveratrol reduced the detrimental effects of malondialdehyde on human endothelial cells. J Cardiovasc Thorac Res 13:131-140 (2021). PubMed: 34326967
- Zhang J et al. Neurotrophin-3 acts on the endothelial-mesenchymal transition of heterotopic ossification in rats. J Cell Mol Med 23:2595-2609 (2019). PubMed: 30672120
- Lipps C et al. Expansion of functional personalized cells with specific transgene combinations. Nat Commun 9:994 (2018). PubMed: 29520052