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リコンビナントRabMAb

Anti-Thyroglobulin 抗体 [CPT-R35.3-31] - BSA and Azide free (ab245205)

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ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
  • Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin - BSA and Azide free
  • Suitable for: IHC-P, ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free
    Thyroglobulin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [CPT-R35.3-31] to Thyroglobulin - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, ELISAmore details
    適用なし: Flow Cyt,ICC/IF or WB

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human thyroid and thyroid cancer tissues. ELISA: Immunizing peptide.

  • 特記事項

    ab245205 is the carrier-free version of ab243093.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    CPT-R35.3-31

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab245205の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or WB.

ターゲット情報

  • 機能

    Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3).

  • 組織特異性

    Thyroid gland specific.

  • 関連疾患

    Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
    Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection.

  • 配列類似性

    Belongs to the type-B carboxylesterase/lipase family.
    Contains 11 thyroglobulin type-1 domains.

  • 翻訳後修飾

    Sulfated tyrosines are desulfated during iodination.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AITD 3 antibody
      • AITD3 antibody
      • hTG antibody
      • TDH3 antibody
      • Tg antibody
      • TGN antibody
      • THYG_HUMAN antibody
      • Thyroglobulin antibody
      see all

    画像

    • ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
      ELISA - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

      50 ng CPTAC-39c (1000 ng/mL, 50μL per well) was coated onto 96-wells. Serial dilutions (0, 0.2, 0.9, 3.9, 15, 62, 250, 1000 ng/mL) of ab243093 (50μL) were incubated with CPTAC-39c in each well for 60 minutes by shaking. This was followed by adding goat anti-rabbit IgG, (H+L), phosphatase-conjugated secondary antibody (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 15 minutes without shaking for color development. OD was read at 405nm within 5 minutes.

      The secondary antibody was used at 1/2500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243093).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

      Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid (PMID: 17381352) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243093).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

      Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative control: No staining on human pancreas (PMID: 10817499) is observed. Counter stained with hematoxylin.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243093).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

      Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue labeling Thyroglobulin with ab243093 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human thyroid cancer (PMID: 3061922) is observed. Counter stained with hematoxylin.
      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243093).
    • Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)
      Anti-Thyroglobulin antibody [CPT-R35.3-31] - BSA and Azide free (ab245205)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab245205 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab245205 は論文での使用が確認できていません。

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