Anti-ThrRS 抗体 [TARSF8H3] (ab50795)
![Western blot - Anti-ThrRS antibody [TARSF8H3] (ab50795)](https://www.abcam.co.jp/ps/products/50/ab50795/Images/ab50795-62488-anti-thrrs-antibody-tarsf8h3-western-blot.jpg "Western blot - Anti-ThrRS antibody [TARSF8H3] (ab50795)")
Key features and details
- Mouse monoclonal [TARSF8H3] to ThrRS
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-ThrRS antibody [TARSF8H3]
ThrRS 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [TARSF8H3] to ThrRS -
由来種
Mouse -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human ThrRS.
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ポジティブ･コントロール
- WB: HeLa whole cell lysate (ab150035). ICC/IF: HeLa cell.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. It is sterile filtered through a 0.22 µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
TARSF8H3 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab50795の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
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| ICC |
1/5.
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| 特記事項 |
|---|
|
WB
1/50. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa). |
|
ICC
1/5. |
ターゲット情報
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配列類似性
Belongs to the class-II aminoacyl-tRNA synthetase family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 6897 Human
- Omim: 187790 Human
- SwissProt: P26639 Human
- Unigene: 481860 Human
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別名
- cytoplasmic antibody
- MGC9344 antibody
- SYTC_HUMAN antibody
see all
画像
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Western blot - Anti-ThrRS antibody [TARSF8H3] (ab50795)Anti-ThrRS antibody [TARSF8H3] (ab50795) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry - Anti-ThrRS antibody [TARSF8H3] (ab50795)](https://www.abcam.co.jp/ps/products/50/ab50795/Images/ab50795-62487-anti-thrrs-antibody-tarsf8h3-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ThrRS antibody [TARSF8H3] (ab50795)Ab50795, at a dilution of 1/5, staining ThrRS in HeLa cell by Immunocytochemistry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab50795 は論文での使用が確認できていません。