Anti-Thrombospondin 1 抗体 [A6.1] (ab1823)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)](https://www.abcam.co.jp/ps/products/1/ab1823/Images/ab1823-275744-ab1823-Supplier-IHC5.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)")
Key features and details
- Mouse monoclonal [A6.1] to Thrombospondin 1
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Thrombospondin 1 antibody [A6.1]
Thrombospondin 1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [A6.1] to Thrombospondin 1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IHC-Pmore details -
種交差性
交差種: Rat, Human -
免疫原
Full length protein corresponding to Human Thrombospondin 1. BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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ポジティブ･コントロール
- Tonsil
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
A6.1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1823の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | (4) |
Use at an assay dependent concentration.
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| IHC-P | (7) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
配列類似性
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
細胞内局在
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
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参照データベース
- Entrez Gene: 7057 Human
- Entrez Gene: 445442 Rat
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
- Unigene: 732539 Human
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別名
- Thbs1 antibody
- Thrombospondin-1 antibody
- TSP antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)
Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.
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![Flow Cytometry - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)](https://www.abcam.co.jp/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-39.jpg)
Flow Cytometry - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)](https://www.abcam.co.jp/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreviewab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)](https://www.abcam.co.jp/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)](https://www.abcam.co.jp/ps/products/1/ab1823/Images/ab1823_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [A6.1] (ab1823)ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (74)
ab1823 は 74 報の論文で使用されています。
- Phan V et al. Molecular mechanisms in chloroquine-exposed muscle cells elucidated by combined proteomic and microscopic studies. Neuropathol Appl Neurobiol 49:e12877 (2023). PubMed: 36633103
- Yin C et al. Suppression of CEBPδ recovers exhaustion in anti-metastatic immune cells. Sci Rep 13:3903 (2023). PubMed: 36890150
- Luo Q et al. Tsp-1+ microglia attenuate retinal neovascularization by maintaining the expression of Smad3 in endothelial cells through exosomes with decreased miR-27a-5p. Theranostics 13:3689-3706 (2023). PubMed: 37441592
- Cheng S et al. Single-cell RNA sequencing reveals the heterogeneity and intercellular communication of hepatic stellate cells and macrophages during liver fibrosis. MedComm (2020) 4:e378 (2023). PubMed: 37724132
- Li B et al. RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice. Elife 11:N/A (2022). PubMed: 35001873