AB227701

Anti-Thrombomodulin 抗体 [SP309] - C-terminal

Anti-Thrombomodulin antibody [SP309] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Thrombomodulin antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

別名を表示する

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human cervical squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human placenta tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human mesothelioma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling Thrombomodulin with ab227701 at 1/300 dilution (green) compared with a rabbit IgG negative control (blue).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

SP309

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p>" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A/G

精製に関する特記事項

Purified from TCS by protein A/G.

バッファー組成

pH: 7.6 Preservative: 0.099% Sodium azide Constituents: PBS, 1% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).

See full target information THBD

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101852 PubMed39657664

2024

Lurbinectedin sensitizes PD-L1 blockade therapy by activating STING-IFN signaling in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Subhamoy Chakraborty,Utsav Sen,Kedwin Ventura,Vrinda Jethalia,Charles Coleman,Subhasree Sridhar,Avisek Banerjee,Hilal Ozakinci,Yazhini Mahendravarman,Konrad Snioch,Elisa de Stanchina,Misty D Shields,Lewis E Tomalin,Deniz Demircioglu,Theresa A Boyle,Anna Tocheva,Dan Hasson,Triparna Sen

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thrombomodulin 抗体 [SP309] - C-terminal

Anti-Thrombomodulin antibody [SP309] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

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Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

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Abcam product promise