Anti-Thrombomodulin 抗体 [Phx-01] (ab24595)
Key features and details
- Mouse monoclonal [Phx-01] to Thrombomodulin
- Suitable for: IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Thrombomodulin antibody [Phx-01]
Thrombomodulin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Phx-01] to Thrombomodulin -
由来種
Mouse -
アプリケーション
適用あり: IHC-Fr, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mammals -
免疫原
Recombinant full length protein (Human)
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エピトープ
This antibody reacts with an epitope within the EGF 5 domain. -
特記事項
ab24595 inhibits thrombin binding.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.03% Thimerosal (merthiolate)
Constituents: 0.09% Tris glycine, PBS -
Concentration information loading...
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精製度
Affinity purified -
一次抗体 備考
ab24595 inhibits thrombin binding. -
ポリ/モノ
モノクローナル -
クローン名
Phx-01 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24595の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr |
1/100.
|
|
WB |
1/500 - 1/1000. Predicted molecular weight: 60 kDa.
|
特記事項 |
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IHC-Fr
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
組織特異性
Endothelial cells are unique in synthesizing thrombomodulin. -
関連疾患
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
翻訳後修飾
N-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7056 Human
- Omim: 188040 Human
- SwissProt: P07204 Human
- Unigene: 2030 Human
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別名
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
画像
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Immunohistochemistry (Frzen sections) of human placenta useing ab24595 at 1/100.
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All lanes : Anti-Thrombomodulin antibody [Phx-01] (ab24595) at 1 µg/ml
Lane 1 : HL-60 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP Goat anti-Mouse secondary antibody at 1/3000 dilution
Predicted band size: 60 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab24595 は 1 報の論文で使用されています。
- Ikezoe T et al. The fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective function and prevents SOS in a murine model. Bone Marrow Transplant 52:73-79 (2017). PubMed: 27427915