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Anti-Thrombomodulin 抗体 [PBS-02] (ab7640)

Reviews (1)Q&A (4)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-02] (ab7640)
  • Flow Cytometry - Anti-Thrombomodulin antibody [PBS-02] (ab7640)

Key features and details

  • Mouse monoclonal [PBS-02] to Thrombomodulin
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Thrombomodulin antibody [PBS-02]
    Thrombomodulin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [PBS-02] to Thrombomodulin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Thrombomodulin.

  • エピトープ

    EGF domain 2 of thrombomodulin.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7640の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use a concentration of 1 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
特記事項
IHC-P
Use a concentration of 1 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.

  • 組織特異性

    Endothelial cells are unique in synthesizing thrombomodulin.

  • 関連疾患

    Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
    Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.

  • 配列類似性

    Contains 1 C-type lectin domain.
    Contains 6 EGF-like domains.

  • 翻訳後修飾

    N-glycosylated.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AHUS 6 antibody
      • AHUS6 antibody
      • BDCA 3 antibody
      • BDCA3 antibody
      • CD 141 antibody
      • CD141 antibody
      • CD141 antigen antibody
      • Fetomodulin antibody
      • Thbd antibody
      • THPH12 antibody
      • THRM antibody
      • Thrombomodulin antibody
      • TM antibody
      • TRBM_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-02] (ab7640)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-02] (ab7640)
      ab7640 (1µg/ml) staining thrmbomodulin in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of pneumocytes.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
    • Flow Cytometry - Anti-Thrombomodulin antibody [PBS-02] (ab7640)
      Flow Cytometry - Anti-Thrombomodulin antibody [PBS-02] (ab7640)
      Overlay histogram showing THP1 cells stained with ab7640 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7640, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献 (2)

    ab7640 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7640 は 2 報の論文で使用されています。

    • Jourdy Y  et al. Why patients with THBD c.1611C>A (p.Cys537X) nonsense mutation have high levels of soluble thrombomodulin? PLoS One 12:e0188213 (2017). PubMed: 29145514
    • Lee EJ & Niklason LE A novel flow bioreactor for in vitro microvascularization. Tissue Eng Part C Methods 16:1191-200 (2010). WB ; Rat . PubMed: 20170423

    レビューと Q&A

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    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-Thrombomodulin antibody [PBS-02]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Human umbilical vein endothelial cells)
    Loading amount
    30 µg
    Specification
    Human umbilical vein endothelial cells
    Gel Running Conditions
    Reduced Denaturing (10%`)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Apr 17 2008

    Question

    Do you sell the recombinant soluble thrombomodulin you used for the standard in your demonstration ELISA?

    Read More
    Answer

    Thank you for your enquiry. We don't sell the truncated recombinant thrombomodulin used as the standard in the ELISA demonstration on the online datasheet. Please contact us again if you require further information to that provided on the datasheet.

    Read More

    Question

    Can you use this antibody for immunohistochemistry using either frozen or paraffin tissues

    Read More
    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

    Read More

    Question

    What is the recommended dilution for western analysis?

    Read More
    Answer

    Try this antibody at 1:1000 and adjust the dilution accordingly from the results obtained.

    Read More

    Question

    Do you if this ab. recognize extracellular domain of throbomodulin. If yes, is it possible to use it for endothelial cell separation via Magnetic cell sorting or FACS

    Read More
    Answer

    Yes, ab7640 antibody recognises EGF domain 2 which is located within the extracellular domain of this integral membrane protein (EGF domains are regions containing sequence homologous to human epidermal growth factor). The antibody has been shown to work in FACS.

    Read More

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